Abstract
Leukemia niche impacts quiescence; however, culturing patient-derived samples ex vivo is technically challenging. Here, we present a protocol for in vitro co-culture of patient-derived xenograft acute lymphoblastic leukemia (PDX-ALL) cells with human mesenchymal stem cells (MSCs). We describe steps for labeling PDX-ALL cells with CellTrace Violet dye to demonstrate MSC-primed PDX-ALL cycling. We then detail procedures to identify MSC-primed G0/quiescent PDX-ALL cells via Hoechst-33342/Pyronin Y live cell cycle analysis.
For complete details on the use and execution of this protocol, please refer to Pal et al.1,2.
| Original language | English |
|---|---|
| Article number | 103202 |
| Pages (from-to) | 1-18 |
| Number of pages | 18 |
| Journal | STAR Protocols |
| Volume | 5 |
| Issue number | 3 |
| Early online date | 20 Jul 2024 |
| DOIs | |
| Publication status | Published - 20 Sept 2024 |
Bibliographical note
Publisher Copyright:© 2024 The Authors
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