Projects per year
Abstract
Surgical treatment of pediatric congenital heart disease with tissue grafts is a lifesaving intervention. Decellularization to reduce immunogenicity of tissue grafts is an increasingly popular alternative to glutaraldehyde fixation. Here, we present a protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber. We describe steps for 3D printing the flow rig, preparing porcine tissue, and using the flow rig to utilize shear forces for decellularization. We then detail procedures for characterizing the acellular scaffold.
For complete details on the use and execution of this protocol, please refer to Vafaee et al.
For complete details on the use and execution of this protocol, please refer to Vafaee et al.
| Original language | English |
|---|---|
| Article number | 102899 |
| Number of pages | 37 |
| Journal | STAR Protocols |
| Volume | 5 |
| Issue number | 1 |
| Early online date | 16 Feb 2024 |
| DOIs | |
| Publication status | Published - 15 Mar 2024 |
Bibliographical note
Publisher Copyright:© 2024 The Author(s).
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Dive into the research topics of 'Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber'. Together they form a unique fingerprint.Projects
- 2 Active
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Symmetry-breaking Technologies for Cerebral Organoid Engineering
Uney, J. B. (Principal Investigator)
1/11/21 → 31/10/25
Project: Research, Parent
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Symmetry-breaking Technologies for Cerebral Organoid Engineering
Drinkwater, B. W. (Principal Investigator)
1/11/21 → 31/10/25
Project: Research
Student theses
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Decellularisation and stem cell tissue engineering of valved right ventricular outflow tract xenografts for correction of tetralogy of Fallot
Harris, A. G. (Author), Caputo, M. (Supervisor), Biglino, G. (Supervisor), Bartoli-Leonard, F. (Supervisor) & Perriman, A. W. (Supervisor), 4 Feb 2025Student thesis: Doctoral Thesis › Doctor of Philosophy (PhD)
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