Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber

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2 Citations (Scopus)

Abstract

Surgical treatment of pediatric congenital heart disease with tissue grafts is a lifesaving intervention. Decellularization to reduce immunogenicity of tissue grafts is an increasingly popular alternative to glutaraldehyde fixation. Here, we present a protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber. We describe steps for 3D printing the flow rig, preparing porcine tissue, and using the flow rig to utilize shear forces for decellularization. We then detail procedures for characterizing the acellular scaffold.

For complete details on the use and execution of this protocol, please refer to Vafaee et al.
Original languageEnglish
Article number102899
Number of pages37
JournalSTAR Protocols
Volume5
Issue number1
Early online date16 Feb 2024
DOIs
Publication statusPublished - 15 Mar 2024

Bibliographical note

Publisher Copyright:
© 2024 The Author(s).

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