Putting the Squeeze on Plasmodesmata: A Role for Reticulons in Primary Plasmodesmata Formation

Kirsten Knox, Pengwei Wang, Verena Kriechbaumer, Jens Tilsner, Lorenzo Frigerio, Imogen Sparkes, Chris Hawes, Karl Oparka

Research output: Contribution to journalArticle (Academic Journal)peer-review

49 Citations (Scopus)

Abstract

Primary plasmodesmata (PD) arise at cytokinesis when the new cell plate forms. During this process, fine strands of endoplasmic reticulum (ER) are laid down between enlarging Golgi-derived vesicles to form nascent PD, each pore containing a desmotubule, a membranous rod derived from the cortical ER. Little is known about the forces that model the ER during cell plate formation. Here, we show that members of the reticulon (RTNLB) family of ER-tubulating proteins in Arabidopsis (Arabidopsis thaliana) may play a role in the formation of the desmotubule. RTNLB3 and RTNLB6, two RTNLBs present in the PD proteome, are recruited to the cell plate at late telophase, when primary PD are formed, and remain associated with primary PD in the mature cell wall. Both RTNLBs showed significant colocalization at PD with the viral movement protein of Tobacco mosaic virus, while superresolution imaging (three-dimensional structured illumination microscopy) of primary PD revealed the central desmotubule to be labeled by RTNLB6. Fluorescence recovery after photobleaching studies showed that these RTNLBs are mobile at the edge of the developing cell plate, where new wall materials are being delivered, but significantly less mobile at its center, where PD are forming. A truncated RTNLB3, unable to constrict the ER, was not recruited to the cell plate at cytokinesis. We discuss the potential roles of RTNLBs in desmotubule formation.

Original languageEnglish
Pages (from-to)1563-72
Number of pages10
JournalPlant Physiology
Volume168
Issue number4
DOIs
Publication statusPublished - Aug 2015

Keywords

  • Arabidopsis Proteins
  • Cell Line
  • Cell Wall
  • Cytokinesis
  • Endoplasmic Reticulum
  • Fluorescence Recovery After Photobleaching
  • Green Fluorescent Proteins
  • Membrane Proteins
  • Microscopy, Confocal
  • Plant Viral Movement Proteins
  • Plants, Genetically Modified
  • Plasmodesmata
  • Protein Transport
  • Tobacco
  • Tobacco Mosaic Virus
  • Journal Article
  • Research Support, Non-U.S. Gov't

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