Quantitative sensing of microviscosity in protocells and amyloid materials using fluorescence lifetime imaging of molecular rotors

Alex J. Thompson*, T D Tang, Therese W. Herling, C. Rohaida Che Hak, Stephen Mann, Tuomas P J Knowles, Marina K. Kuimova

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference Contribution (Conference Proceeding)

2 Citations (Scopus)

Abstract

Molecular rotors are fluorophores that have a fluorescence quantum yield that depends upon intermolecular rotation. The fluorescence quantum yield, intensity and lifetime of molecular rotors all vary as functions of viscosity, as high viscosities inhibit intermolecular rotation and cause an increase in the non-radiative decay rate. As such, molecular rotors can be used to probe viscosity on microscopic scales. Here, we apply fluorescence lifetime imaging microscopy (FLIM) to measure the fluorescence lifetimes of three different molecular rotors, in order to determine the microscopic viscosity in two model systems with significant biological interest. First, the constituents of a novel protocell - a model of a prebiotic cell - were studied using the molecular rotors BODIPY C10 and kiton red. Second, amyloid formation was investigated using the molecular rotor Cy3.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSociety of Photo-Optical Instrumentation Engineers (SPIE)
Volume8947
ISBN (Print)9780819498601
DOIs
Publication statusPublished - 1 Jan 2014
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII - San Francisco, CA, United Kingdom
Duration: 3 Feb 20146 Feb 2014

Conference

ConferenceImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
CountryUnited Kingdom
CitySan Francisco, CA
Period3/02/146/02/14

Keywords

  • Amyloid
  • Confocal microscopy
  • Flim
  • Fluorescence
  • Fluorescence lifetime
  • Lysozyme
  • Molecular rotor
  • Neurodegeneration
  • Protein aggregation
  • Protocells
  • Viscosity

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