Query-Guided Protein-Protein Interaction Inhibitor Discovery

Gail J Bartlett, Amaurys Avila Ibarra, Deborah K Shoemark, Dek N Woolfson, Richard B Sessions, David Andrews*, Adam Nelson*, Andrew J. Wilson*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

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Abstract

Protein–protein interactions (PPIs) are central to biological mechanisms, and can serve as compelling targets for drug discovery. Yet, the discovery of small molecule inhibitors of PPIs remains challenging given the large and typically shallow topography of the interacting protein surfaces. Here, we describe a general approach to the discovery of orthosteric PPI inhibitors that mimic specific secondary protein structures. Initially, hot residues at protein–protein interfaces are identified in silico or from experimental data, and incorporated into secondary structure-based queries. Virtual libraries of small molecules are then shape-matched against the queries, and promising ligands docked to target proteins. The approach is exemplified experimentally using two unrelated PPIs that are mediated by an α-helix (p53/hDM2) and a β-strand (GKAP/SHANK1-PDZ). In each case, selective PPI inhibitors are discovered with low μM activity as determined by a combination of fluorescence anisotropy and 1H–15N HSQC experiments. In addition, hit expansion yields a series of PPI inhibitors with defined structure–activity relationships. It is envisaged that the generality of the approach will enable discovery of inhibitors of a wide range of unrelated secondary structure-mediated PPIs.
Original languageEnglish
Pages (from-to)4753-4762
Number of pages10
JournalChemical Science
Volume12
Issue number13
Early online date2 Mar 2021
DOIs
Publication statusPublished - 7 Apr 2021

Bibliographical note

Funding Information:
This work was supported by EPSRC (EP/N013573/1 and EP/ KO39292/1) and the BBSRC/EPSRC-funded Synthetic Biology Research Centre, BrisSynBio (BB/L01386X/1). AJW holds a Royal Society Leverhulme Trust Senior Fellowship (SRF/R1/191087), AN holds an EPSRC Established Career Fellowship (EP/ N025652/1). The authors wish to thank Kirsten Spence and Pallavi Ramsahye for protein production. We acknowledge Diamond Light Source for time on Beamline I03, I04 and I24 under Proposal mx19248 and the authors would like to thank the staff of these beamlines for assistance with data collection.

Publisher Copyright:
© The Royal Society of Chemistry 2021.

Structured keywords

  • BrisSynBio
  • Bristol BioDesign Institute

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