Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants

Imogen A Sparkes, John Runions, Anne Kearns, Chris Hawes

Research output: Contribution to journalArticle (Academic Journal)peer-review

909 Citations (Scopus)

Abstract

Expression and tracking of fluorescent fusion proteins has revolutionized our understanding of basic concepts in cell biology. The protocol presented here has underpinned much of the in vivo results highlighting the dynamic nature of the plant secretory pathway. Transient transformation of tobacco leaf epidermal cells is a relatively fast technique to assess expression of genes of interest. These cells can be used to generate stable plant lines using a more time-consuming, cell culture technique. Transient expression takes from 2 to 4 days whereas stable lines are generated after approximately 2 to 4 months.

Original languageEnglish
Pages (from-to)2019-25
Number of pages7
JournalNature Protocols
Volume1
Issue number4
DOIs
Publication statusPublished - 2006

Keywords

  • Cloning, Molecular
  • Gene Expression
  • Gene Transfer Techniques
  • Green Fluorescent Proteins
  • Recombinant Fusion Proteins
  • Rhizobium
  • Tissue Culture Techniques
  • Tobacco
  • Journal Article
  • Research Support, Non-U.S. Gov't

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