Ras controls tumor necrosis factor receptor-associated factor (TRAF)6-dependent induction of nuclear factor-kappa B. Selective regulation through receptor signaling components

CJ Caunt, E. Kiss-Toth, F. Carlotti, R. Chapman, EE Qwarnstrom

    Research output: Contribution to journalArticle (Academic Journal)peer-review

    25 Citations (Scopus)

    Abstract

    In the present study, we show that Ras activity differentially controls interleukin (IL)-1 induced transcription factor activation by selective regulation of responses mediated by receptor complex components. Initial experiments revealed that stimulation with IL-1 caused a rapid, matrix-dependent activation of Ras. The effect was transient, peaking at 5 min and returning to base levels after 30 min. Activation correlated with pronounced changes in cell shape in EGFPH-Ras transfected cells. Transfection with the dominant negative mutant, RasAsn-17, inhibited IL-1 induced activation of the IL-8 promoter as well as of NF-kappa B and AP-1 synthetic promoters in transient transfection assays. Furthermore, overexpression of the IL-1 signaling proteins TRAF6 or MyD88 gave characteristic activation of IL-8, which was accentuated in the presence of IL-1. Co-transfection with RasAsn-17 gave a dose-dependent inhibition of TRAF6-induced responses in the presence and absence of IL-1, but had no effect on MyD88 mediated activity. Similarly, induction of NF-kappa B was abolished by RasAsn-17 only in TRAF6-transfected cells. In contrast, inhibiting Ras activity limited AP-1-mediated responses through both receptor complex proteins. Constitutively active RasVal-12 increased the TRAF6 induced activity of the NF-kappa B pathway similar to the effect induced by IL-1, while the RasVal-12 induced activity was not inhibited by co-transfection with a dominant negative TRAF6. Our data show that activation of the Ras GTPase is an early, matrix-dependent response in IL-1 signaling which participates in structural regulation of IL-1-induced genes. In addition, they show that the Ras induced effect selectively regulates TRAF6-mediated activation of the NF-kappa B pathway, suggesting that Ras GTPase represents a convergence point in structural and cytokine responses, with distinct effects on a subset of downstream signaling events.
    Translated title of the contributionRas controls tumor necrosis factor receptor-associated factor (TRAF)6-dependent induction of nuclear factor-kappa B. Selective regulation through receptor signaling components
    Original languageEnglish
    Pages (from-to)6280 - 6288
    Number of pages9
    JournalJournal of Biological Chemistry
    Volume276
    DOIs
    Publication statusPublished - Mar 2001

    Bibliographical note

    Author of Publication Reviewed: Caunt CJ, Kiss-Toth E, Carlotti F, Chapman R & Qwarnstrom EE

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