Reactive oxygen species modulate the barrier function of the human glomerular endothelial glycocalyx

Anurag Singh, Raina D Ramnath, Rebecca R Foster, Emma C Wylie, Vincent Fridén, Ishita Dasgupta, Borje Haraldsson, Gavin I Welsh, Peter W Mathieson, Simon C Satchell

Research output: Contribution to journalArticle (Academic Journal)peer-review

107 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) play a key role in the pathogenesis of proteinuria in glomerular diseases like diabetic nephropathy. Glomerular endothelial cell (GEnC) glycocalyx covers the luminal aspect of the glomerular capillary wall and makes an important contribution to the glomerular barrier. ROS are known to depolymerise glycosaminoglycan (GAG) chains of proteoglycans, which are crucial for the barrier function of GEnC glycocalyx. The aim of this study is to investigate the direct effects of ROS on the structure and function of GEnC glycocalyx using conditionally immortalised human GEnC. ROS were generated by exogenous hydrogen peroxide. Biosynthesis and cleavage of GAG chains was analyzed by radiolabelling (S(35) and (3)H-glucosamine). GAG chains were quantified on GEnC surface and in the cell supernatant using liquid chromatography and immunofluorescence techniques. Barrier properties were estimated by measuring trans-endothelial passage of albumin. ROS caused a significant loss of WGA lectin and heparan sulphate staining from the surface of GEnC. This lead to an increase in trans-endothelial albumin passage. The latter could be inhibited by catalase and superoxide dismutase. The effect of ROS on GEnC was not mediated via the GAG biosynthetic pathway. Quantification of radiolabelled GAG fractions in the supernatant confirmed that ROS directly caused shedding of HS GAG. This finding is clinically relevant and suggests a mechanism by which ROS may cause proteinuria in clinical conditions associated with high oxidative stress.

Original languageEnglish
Pages (from-to)e55852
JournalPLoS ONE
Volume8
Issue number2
DOIs
Publication statusPublished - 2013

Keywords

  • Albumins
  • Cell Line
  • Cell Survival
  • Endothelial Cells
  • Glycocalyx
  • Glycosaminoglycans
  • Humans
  • Kidney Glomerulus
  • Reactive Oxygen Species
  • Wheat Germ Agglutinins

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    Project: Research

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