Recent insights into eukaryotic double-strand DNA break repair unveiled by single-molecule methods

Sara de Bragança; Mark S Dillingham; Fernando Moreno-Herrero

doi:10.1016/j.tig.2023.09.004

Recent insights into eukaryotic double-strand DNA break repair unveiled by single-molecule methods

Sara de Bragança, Mark S Dillingham, Fernando Moreno-Herrero^*

^*Corresponding author for this work

School of Biochemistry

Research output: Contribution to journal › Article (Academic Journal) › peer-review

5 Citations (Scopus)

Abstract

Genome integrity and maintenance are essential for the viability of all organisms. A wide variety of DNA damage types have been described, but double-strand breaks (DSBs) stand out as one of the most toxic DNA lesions. Two major pathways account for the repair of DSBs: homologous recombination (HR) and non-homologous end joining (NHEJ). Both pathways involve complex DNA transactions catalyzed by proteins that sequentially or cooperatively work to repair the damage. Single-molecule methods allow visualization of these complex transactions and characterization of the protein:DNA intermediates of DNA repair, ultimately allowing a comprehensive breakdown of the mechanisms underlying each pathway. We review current understanding of the HR and NHEJ responses to DSBs in eukaryotic cells, with a particular emphasis on recent advances through the use of single-molecule techniques.

Original language	English
Pages (from-to)	924-940
Number of pages	17
Journal	Trends in genetics
Volume	39
Issue number	12
DOIs	https://doi.org/10.1016/j.tig.2023.09.004
Publication status	Published - 6 Oct 2023

Bibliographical note

Funding Information:
We would like to acknowledge the many single-molecule studies that could not be addressed in this review due to space limitations. Work in the laboratory of F.M-H. was supported by grants PID2020-112998GB-I00 from the Ministerio de Ciencia e Innovación (MICINN)/ Agencia Estatal de Investigación ( AEI/10.13039/501100011033 )_FEDER, EU, cofunded by the European Regional Development Fund (ERDF); grants Y2018/BIO4747 and P2018/NMT4443 from the Autonomous Region of Madrid and cofunded by the European Social Fund (ESF) and the ERDF; and grant EUREXCEL 951214 funded by CSIC. Work in the laboratory of M.S.D. was supported by the Wellcome Trust ( 100401/Z/12/Z ).

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© 2023 The Author(s)

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title = "Recent insights into eukaryotic double-strand DNA break repair unveiled by single-molecule methods",

abstract = "Genome integrity and maintenance are essential for the viability of all organisms. A wide variety of DNA damage types have been described, but double-strand breaks (DSBs) stand out as one of the most toxic DNA lesions. Two major pathways account for the repair of DSBs: homologous recombination (HR) and non-homologous end joining (NHEJ). Both pathways involve complex DNA transactions catalyzed by proteins that sequentially or cooperatively work to repair the damage. Single-molecule methods allow visualization of these complex transactions and characterization of the protein:DNA intermediates of DNA repair, ultimately allowing a comprehensive breakdown of the mechanisms underlying each pathway. We review current understanding of the HR and NHEJ responses to DSBs in eukaryotic cells, with a particular emphasis on recent advances through the use of single-molecule techniques.",

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note = "Funding Information: We would like to acknowledge the many single-molecule studies that could not be addressed in this review due to space limitations. Work in the laboratory of F.M-H. was supported by grants PID2020-112998GB-I00 from the Ministerio de Ciencia e Innovaci{\'o}n (MICINN)/ Agencia Estatal de Investigaci{\'o}n ( AEI/10.13039/501100011033 )_FEDER, EU, cofunded by the European Regional Development Fund (ERDF); grants Y2018/BIO4747 and P2018/NMT4443 from the Autonomous Region of Madrid and cofunded by the European Social Fund (ESF) and the ERDF; and grant EUREXCEL 951214 funded by CSIC. Work in the laboratory of M.S.D. was supported by the Wellcome Trust ( 100401/Z/12/Z ). Publisher Copyright: {\textcopyright} 2023 The Author(s)",

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N1 - Funding Information: We would like to acknowledge the many single-molecule studies that could not be addressed in this review due to space limitations. Work in the laboratory of F.M-H. was supported by grants PID2020-112998GB-I00 from the Ministerio de Ciencia e Innovación (MICINN)/ Agencia Estatal de Investigación ( AEI/10.13039/501100011033 )_FEDER, EU, cofunded by the European Regional Development Fund (ERDF); grants Y2018/BIO4747 and P2018/NMT4443 from the Autonomous Region of Madrid and cofunded by the European Social Fund (ESF) and the ERDF; and grant EUREXCEL 951214 funded by CSIC. Work in the laboratory of M.S.D. was supported by the Wellcome Trust ( 100401/Z/12/Z ). Publisher Copyright: © 2023 The Author(s)

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N2 - Genome integrity and maintenance are essential for the viability of all organisms. A wide variety of DNA damage types have been described, but double-strand breaks (DSBs) stand out as one of the most toxic DNA lesions. Two major pathways account for the repair of DSBs: homologous recombination (HR) and non-homologous end joining (NHEJ). Both pathways involve complex DNA transactions catalyzed by proteins that sequentially or cooperatively work to repair the damage. Single-molecule methods allow visualization of these complex transactions and characterization of the protein:DNA intermediates of DNA repair, ultimately allowing a comprehensive breakdown of the mechanisms underlying each pathway. We review current understanding of the HR and NHEJ responses to DSBs in eukaryotic cells, with a particular emphasis on recent advances through the use of single-molecule techniques.

AB - Genome integrity and maintenance are essential for the viability of all organisms. A wide variety of DNA damage types have been described, but double-strand breaks (DSBs) stand out as one of the most toxic DNA lesions. Two major pathways account for the repair of DSBs: homologous recombination (HR) and non-homologous end joining (NHEJ). Both pathways involve complex DNA transactions catalyzed by proteins that sequentially or cooperatively work to repair the damage. Single-molecule methods allow visualization of these complex transactions and characterization of the protein:DNA intermediates of DNA repair, ultimately allowing a comprehensive breakdown of the mechanisms underlying each pathway. We review current understanding of the HR and NHEJ responses to DSBs in eukaryotic cells, with a particular emphasis on recent advances through the use of single-molecule techniques.

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JO - Trends in genetics

JF - Trends in genetics

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ER -

Recent insights into eukaryotic double-strand DNA break repair unveiled by single-molecule methods

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