Recombination hotspots attenuate the coupled ATPase and translocase activities of an AddAB-type helicase-nuclease

Neville S Gilhooly, Mark S Dillingham

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

In all domains of life, the resection of double-stranded DNA breaks to form long 3'-ssDNA overhangs in preparation for recombinational repair is catalyzed by the coordinated activities of DNA helicases and nucleases. In bacterial cells, this resection reaction is modulated by the recombination hotspot sequence Chi. The Chi sequence is recognized in cis by translocating helicase-nuclease complexes such as the Bacillus subtilis AddAB complex. Binding of Chi to AddAB results in the attenuation of nuclease activity on the 3'-terminated strand, thereby promoting recombination. In this work, we used stopped-flow methods to monitor the coupling of adenosine triphosphate (ATP) hydrolysis and DNA translocation and how this is affected by Chi recognition. We show that in the absence of Chi sequences, AddAB translocates processively on DNA at ∼2000 bp s(-1) and hydrolyses approximately 1 ATP molecule per base pair travelled. The recognition of recombination hotspots results in a sustained decrease in the translocation rate which is accompanied by a decrease in the ATP hydrolysis rate, such that the coupling between these activities and the net efficiency of DNA translocation is largely unchanged by Chi.

Original languageEnglish
JournalNucleic Acids Research
DOIs
Publication statusPublished - 15 Mar 2014

Fingerprint Dive into the research topics of 'Recombination hotspots attenuate the coupled ATPase and translocase activities of an AddAB-type helicase-nuclease'. Together they form a unique fingerprint.

Cite this