Abstract
The E28D variant of dihydrofolate reductase from Moritella profunda was generated and found to have the same K i (within error) for the competitive inhibitor trimethoprim as the wild type enzyme. Contrary to a previous claim in the literature, Glu 28 is therefore not the cause of the reduced affinity for trimethoprim relative to dihydrofolate reductase from Escherichia coli.
Original language | English |
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Pages (from-to) | 546-548 |
Number of pages | 3 |
Journal | Protein Journal |
Volume | 30 |
Issue number | 8 |
DOIs | |
Publication status | Published - 1 Dec 2011 |
Keywords
- Dihydrofolate reductase
- Inhibition
- Moritella profunda
- Trimethoprim