Reduced susceptibility of moritella profunda dihydrofolate reductase to trimethoprim is not due to glutamate 28

E. Joel Loveridge; William M. Dawson; Rhiannon M. Evans; Anna Sobolewska; Rudolf K. Allemann

doi:10.1007/s10930-011-9361-x

Reduced susceptibility of moritella profunda dihydrofolate reductase to trimethoprim is not due to glutamate 28

E. Joel Loveridge, William M. Dawson, Rhiannon M. Evans, Anna Sobolewska, Rudolf K. Allemann^*

^*Corresponding author for this work

School of Chemistry

Research output: Contribution to journal › Article (Academic Journal) › peer-review

2 Citations (Scopus)

Abstract

The E28D variant of dihydrofolate reductase from Moritella profunda was generated and found to have the same K _i (within error) for the competitive inhibitor trimethoprim as the wild type enzyme. Contrary to a previous claim in the literature, Glu 28 is therefore not the cause of the reduced affinity for trimethoprim relative to dihydrofolate reductase from Escherichia coli.

Original language	English
Pages (from-to)	546-548
Number of pages	3
Journal	Protein Journal
Volume	30
Issue number	8
DOIs	https://doi.org/10.1007/s10930-011-9361-x
Publication status	Published - 1 Dec 2011

Keywords

Dihydrofolate reductase
Inhibition
Moritella profunda
Trimethoprim

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title = "Reduced susceptibility of moritella profunda dihydrofolate reductase to trimethoprim is not due to glutamate 28",

abstract = "The E28D variant of dihydrofolate reductase from Moritella profunda was generated and found to have the same K i (within error) for the competitive inhibitor trimethoprim as the wild type enzyme. Contrary to a previous claim in the literature, Glu 28 is therefore not the cause of the reduced affinity for trimethoprim relative to dihydrofolate reductase from Escherichia coli.",

keywords = "Dihydrofolate reductase, Inhibition, Moritella profunda, Trimethoprim",

author = "Loveridge, {E. Joel} and Dawson, {William M.} and Evans, {Rhiannon M.} and Anna Sobolewska and Allemann, {Rudolf K.}",

year = "2011",

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doi = "10.1007/s10930-011-9361-x",

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journal = "Protein Journal",

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T1 - Reduced susceptibility of moritella profunda dihydrofolate reductase to trimethoprim is not due to glutamate 28

AU - Loveridge, E. Joel

AU - Dawson, William M.

AU - Evans, Rhiannon M.

AU - Sobolewska, Anna

AU - Allemann, Rudolf K.

PY - 2011/12/1

Y1 - 2011/12/1

N2 - The E28D variant of dihydrofolate reductase from Moritella profunda was generated and found to have the same K i (within error) for the competitive inhibitor trimethoprim as the wild type enzyme. Contrary to a previous claim in the literature, Glu 28 is therefore not the cause of the reduced affinity for trimethoprim relative to dihydrofolate reductase from Escherichia coli.

AB - The E28D variant of dihydrofolate reductase from Moritella profunda was generated and found to have the same K i (within error) for the competitive inhibitor trimethoprim as the wild type enzyme. Contrary to a previous claim in the literature, Glu 28 is therefore not the cause of the reduced affinity for trimethoprim relative to dihydrofolate reductase from Escherichia coli.

KW - Dihydrofolate reductase

KW - Inhibition

KW - Moritella profunda

KW - Trimethoprim

UR - http://www.scopus.com/inward/record.url?scp=84855945039&partnerID=8YFLogxK

U2 - 10.1007/s10930-011-9361-x

DO - 10.1007/s10930-011-9361-x

M3 - Article (Academic Journal)

C2 - 21968646

AN - SCOPUS:84855945039

SN - 1572-3887

VL - 30

SP - 546

EP - 548

JO - Protein Journal

JF - Protein Journal

IS - 8

ER -

Reduced susceptibility of moritella profunda dihydrofolate reductase to trimethoprim is not due to glutamate 28

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