Reduction of Influenza Virus Envelope's Fusogenicity by Viral Fusion Inhibitors

Michael Rowse, Shihong Qiu, Jun Tsao, Yohei Yamauchi, Guoxin Wang, Ming Luo

Research output: Contribution to journalArticle (Academic Journal)

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Abstract

During cell entry of an enveloped virus, the viral membrane must be fused with the cellular membrane. The virus envelope has a unique structure consisting of viral proteins and a virus-specific lipid composition, whereas the host membrane has its own structure with host membrane proteins. Compound 136 was previously found to bind in close proximity to the viral envelope and inhibit influenza virus entry. We showed here that the 136-treated influenza virus still caused hemolysis. When liposomes were used as the target membrane for 136-treated viruses, aberrant fusion occurred; few liposomes fused per virion, and glycoproteins were not distributed evenly across fusion complexes. Additionally, large fusion aggregates did not form, and in some instances, neck-like structures were found. Based on previous results and hemolysis, fusion inhibition by 136 occurs post-scission but prior to lipid mixing.

Original languageEnglish
Pages (from-to)47-53
Number of pages7
JournalACS Infectious Diseases
Volume2
Issue number1
Early online date15 Oct 2015
DOIs
Publication statusPublished - 8 Jan 2016

Keywords

  • lipid composition
  • liposome
  • fusogenicity
  • influenza virus
  • fusion inhibitor

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