Skip to content

Re-epithelialization of cutaneous wounds in adult zebrafish combines mechanisms of wound closure in embryonic and adult mammals

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)2077-2088
Number of pages12
JournalDevelopment (Cambridge)
Volume143
Early online date27 Apr 2016
DOIs
DateAccepted/In press - 12 Apr 2016
DateE-pub ahead of print - 27 Apr 2016
DatePublished (current) - 15 Jun 2016

Abstract

Re-epithelialization of cutaneous wounds in adult mammals takes days to complete and relies on numerous signalling cues and multiple overlapping cellular processes that take place both within the epidermis and in other participating tissues. Re-epithelialization of partial- or full-thickness skin wounds of adult zebrafish, however, is extremely rapid and largely independent of the other processes of wound healing. Live imaging after treatment with transgene-encoded or chemical inhibitors reveals that re-epithelializing keratinocytes repopulate wounds by TGFβ- and integrin-dependent lamellipodial crawling at the leading edges of the epidermal tongue. In addition, re-epithelialization requires long-range Rho kinase-, JNK- and, to some extent, planar cell polarity-dependent epithelial rearrangements within the following epidermis, involving radial intercalations, flattening and directed elongations of cells. These rearrangements lead to a massive recruitment of keratinocytes from the adjacent epidermis and make re-epithelialization independent of keratinocyte proliferation and the mitogenic effect of FGF signalling, which are only required after wound closure, allowing the epidermis outside the wound to re-establish its normal thickness. Together these results demonstrate that the adult zebrafish is a valuable in-vivo model for studying, and visualizing, the processes involved in cutaneous wound closure, facilitating the dissection of direct from indirect, and motogenic from mitogenic effects of genes and molecules affecting wound re-epithelialization.

    Research areas

  • Zebrafish, Skin, Wound Healing, Re-epithelialization, Live imaging

Download statistics

No data available

Documents

Documents

  • dev.130492.full

    Rights statement: This is the final published version of the article (version of record). It first appeared online via Company of Biologists at http://dx.doi.org/10.1242/dev.130492.

    Final published version, 2.2 MB, PDF document

DOI

View research connections

Related faculties, schools or groups