Regulation and Rate Enhancement during Transcription-Coupled DNA Repair

L Manelyte, Y-IT Kim, A.J Smith, R.M Smith, N.J Savery

Research output: Contribution to journalArticle (Academic Journal)peer-review

41 Citations (Scopus)

Abstract

Transcription-coupled DNA repair (TCR) is a subpathway of nucleotide excision repair (NER) that is triggered when RNA polymerase is stalled by DNA damage. Lesions targeted by TCR are repaired more quickly than lesions repaired by the transcription-independent “global” NER pathway, but the mechanism underlying this rate enhancement is not understood. Damage recognition during bacterial NER depends upon UvrA, which binds to the damage and loads UvrB onto the DNA. Bacterial TCR additionally requires the Mfd protein, a DNA translocase that removes the stalled transcription complexes. We have determined the properties of Mfd, UvrA, and UvrB that are required for the elevated rate of repair observed during TCR. We show that TCR and global NER differ in their requirements for damage recognition by UvrA, indicating that Mfd acts at the very earliest stage of the repair process and extending the functional similarities between TCR in bacteria and eukaryotes.
Translated title of the contributionRegulation and rate enhancement during transcription-coupled DNA repair
Original languageEnglish
Pages (from-to)714 - 724
Number of pages11
JournalMolecular Cell
Volume40
Issue number5
DOIs
Publication statusPublished - Dec 2010

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