Regulation of Scaffold Cell Adhesion Using Artificial Membrane Binding Proteins

Madeline Burke, James P Armstrong, Andrew Goodwin, Robert C Deller, Ben M Carter, Robert L Harniman, Aasiya Ginwalla, Valeska Ting, Sean A Davis, Adam W Perriman

Research output: Contribution to journalArticle (Academic Journal)

4 Citations (Scopus)
320 Downloads (Pure)

Abstract

The rapid pace of development in biotechnology has placed great importance on controlling cell-material interactions. In practice, this involves attempting to decouple the contributions from adhesion molecules, cell membrane receptors, and scaffold surface chemistry and morphology, which is extremely challenging. Accordingly, a strategy is presented in which different chemical, biochemical, and morphological properties of 3D biomaterials are systematically varied to produce novel scaffolds with tuneable cell affinities. Specifically, cationized and surfactant-conjugated proteins, recently shown to have non-native membrane affinity, are covalently attached to 3D scaffolds of collagen or carboxymethyl-dextran, yielding surface-functionalized 3D architectures with predictable cell immobilization profiles. The artificial membrane-binding proteins enhance cellular adhesion of human mesenchymal stem cells (hMSCs) via electrostatic and hydrophobic binding mechanisms. Furthermore, functionalizing the 3D scaffolds with cationized or surfactant-conjugated myoglobin prevents a slowdown in proliferation of seeded hMSCs cultured for seven days under hypoxic conditions.

Original languageEnglish
Article number1600523
Number of pages9
JournalMacromolecular Bioscience
Volume17
Issue number7
Early online date24 Feb 2017
DOIs
Publication statusPublished - Jul 2017

Keywords

  • cellular adhesion
  • hypoxia
  • myoglobin
  • scaffolds
  • stem cells

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