Projects per year
Abstract
The rapid pace of development in biotechnology has placed great importance on controlling cell-material interactions. In practice, this involves attempting to decouple the contributions from adhesion molecules, cell membrane receptors, and scaffold surface chemistry and morphology, which is extremely challenging. Accordingly, a strategy is presented in which different chemical, biochemical, and morphological properties of 3D biomaterials are systematically varied to produce novel scaffolds with tuneable cell affinities. Specifically, cationized and surfactant-conjugated proteins, recently shown to have non-native membrane affinity, are covalently attached to 3D scaffolds of collagen or carboxymethyl-dextran, yielding surface-functionalized 3D architectures with predictable cell immobilization profiles. The artificial membrane-binding proteins enhance cellular adhesion of human mesenchymal stem cells (hMSCs) via electrostatic and hydrophobic binding mechanisms. Furthermore, functionalizing the 3D scaffolds with cationized or surfactant-conjugated myoglobin prevents a slowdown in proliferation of seeded hMSCs cultured for seven days under hypoxic conditions.
Original language | English |
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Article number | 1600523 |
Number of pages | 9 |
Journal | Macromolecular Bioscience |
Volume | 17 |
Issue number | 7 |
Early online date | 24 Feb 2017 |
DOIs | |
Publication status | Published - Jul 2017 |
Keywords
- cellular adhesion
- hypoxia
- myoglobin
- scaffolds
- stem cells
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Dive into the research topics of 'Regulation of Scaffold Cell Adhesion Using Artificial Membrane Binding Proteins'. Together they form a unique fingerprint.Projects
- 3 Finished
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Supercharged enzyme-polymer surfactant bioblocks for the preparation of organophosphate decontaminating materials
Perriman, A. W. (Principal Investigator)
1/05/16 → 21/12/19
Project: Research
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Photo-oxidation and cryofluorescence for Correlative Light Electron Microscopy
Stephens, D. J. (Principal Investigator)
1/12/13 → 1/12/16
Project: Research
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Functional Biomolecular Liquids
Perriman, A. W. (Principal Investigator)
1/10/13 → 30/09/18
Project: Research
Equipment
Profiles
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Dr Sean A Davis
- School of Chemistry - Senior Lecturer
- Soft Matter, Colloids and Materials
Person: Academic , Member