Repair of meniscal cartilage white zone tears using a stem cell/collagen-scaffold implant

Moreica B Pabbruwe, Wael Kafienah, John F Tarlton, Sanjay Mistry, Dennis J Fox, Anthony P Hollander

Research output: Contribution to journalArticle (Academic Journal)peer-review

93 Citations (Scopus)

Abstract

Injuries to the avascular region of knee meniscal cartilage do not heal spontaneously. To address this problem we have developed a new stem cell/collagen-scaffold implant system in which human adult bone marrow mesenchymal stem cells are seeded onto a biodegradable scaffold that allows controlled delivery of actively dividing cells to the meniscus surface. Sandwich constructs of two white zone ovine meniscus discs with stem cell/collagen-scaffold implant in between were cultured in vitro for 40 days. Histomorphometric analysis revealed superior integration in the stem cell/collagen-scaffold groups compared to the cell-free collagen membrane or untreated controls. The addition of TGF-beta1 to differentiate stem cells to chondrocytes inhibited integration. Biomechanical testing demonstrated a significant 2-fold increase in tensile strength in all constructs using the stem cell/collagen-scaffold compared to control groups after 40 days in culture. Integration was significantly higher when collagen membranes were used that had a more open/spongy structure adjacent to both meniscal cartilage surfaces, whereas a collagen scaffold designed for osteoinduction failed to induce any integration of meniscus. In conclusion, the stem cell/collagen-scaffold implant is a potential therapeutic treatment for the repair of white zone meniscal cartilage tears.
Translated title of the contributionRepair of meniscal cartilage white zone tears using a stem cell/collagen-scaffold implant
Original languageEnglish
Pages (from-to)2583 - 2591
Number of pages9
JournalBiomaterials
Volume31
Issue number9
DOIs
Publication statusPublished - Mar 2010

Bibliographical note

Publisher: Elsevier

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