Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells

Laura De Girolamo; Stefania Niada; Elena Arrigoni; Alessia Di Giancamillo; Cinzia Domeneghini; Mahrokh Dadsetan; Michael J. Yaszemski; Dario Gastaldi; Pasquale Vena; Matteo Taffetani; Alberto Zerbi; Valerio Sansone; Giuseppe M. Peretti; Anna T. Brini

doi:10.2217/rme.14.77

Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells

Laura De Girolamo^*, Stefania Niada, Elena Arrigoni, Alessia Di Giancamillo, Cinzia Domeneghini, Mahrokh Dadsetan, Michael J. Yaszemski, Dario Gastaldi, Pasquale Vena, Matteo Taffetani, Alberto Zerbi, Valerio Sansone, Giuseppe M. Peretti, Anna T. Brini

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

38 Citations (Scopus)

Abstract

Aim: Critical knee osteochondral defects in seven adult minipigs were treated with oligo(polyethylene glycol)fumarate (OPF) hydrogel combined with autologous or human adipose-derived stem cells (ASCs), and evaluated after 6 months. Methods: Four defects were made on the peripheral part of right trochleas (n = 28), and treated with OPF scaffold alone or pre-seeded with ASCs. Results: A better quality cartilage tissue characterized by improved biomechanical properties and higher collagen type II expression was observed in the defects treated by autologous or human ASC-loaded OPF; similarly this approach induced the regeneration of more mature bone with upregulation of collagen type I expression. Conclusion: This study provides the evidence that both porcine and human adipose-derived stem cells associated to OPF hydrogel allow improving osteochondral defect regeneration in a minipig model.

Original language	English
Pages (from-to)	135-151
Number of pages	17
Journal	Regenerative Medicine
Volume	10
Issue number	2
DOIs	https://doi.org/10.2217/rme.14.77
Publication status	Published - 1 Mar 2015

Bibliographical note

Publisher Copyright:
© 2015 Future Medicine Ltd.

Keywords

adipose-derived stem cells
bone
cartilage
oligo(polyethylene glycol)fumarate hydrogel
osteochondral defects

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De Girolamo, L., Niada, S., Arrigoni, E., Di Giancamillo, A., Domeneghini, C., Dadsetan, M., Yaszemski, M. J., Gastaldi, D., Vena, P., Taffetani, M., Zerbi, A., Sansone, V., Peretti, G. M., & Brini, A. T. (2015). Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells. Regenerative Medicine, 10(2), 135-151. https://doi.org/10.2217/rme.14.77

@article{03d261fd463f4ef5926682ab4d5031f3,

title = "Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells",

abstract = "Aim: Critical knee osteochondral defects in seven adult minipigs were treated with oligo(polyethylene glycol)fumarate (OPF) hydrogel combined with autologous or human adipose-derived stem cells (ASCs), and evaluated after 6 months. Methods: Four defects were made on the peripheral part of right trochleas (n = 28), and treated with OPF scaffold alone or pre-seeded with ASCs. Results: A better quality cartilage tissue characterized by improved biomechanical properties and higher collagen type II expression was observed in the defects treated by autologous or human ASC-loaded OPF; similarly this approach induced the regeneration of more mature bone with upregulation of collagen type I expression. Conclusion: This study provides the evidence that both porcine and human adipose-derived stem cells associated to OPF hydrogel allow improving osteochondral defect regeneration in a minipig model.",

keywords = "adipose-derived stem cells, bone, cartilage, oligo(polyethylene glycol)fumarate hydrogel, osteochondral defects",

author = "\{De Girolamo\}, Laura and Stefania Niada and Elena Arrigoni and \{Di Giancamillo\}, Alessia and Cinzia Domeneghini and Mahrokh Dadsetan and Yaszemski, \{Michael J.\} and Dario Gastaldi and Pasquale Vena and Matteo Taffetani and Alberto Zerbi and Valerio Sansone and Peretti, \{Giuseppe M.\} and Brini, \{Anna T.\}",

note = "Publisher Copyright: {\textcopyright} 2015 Future Medicine Ltd.",

year = "2015",

month = mar,

day = "1",

doi = "10.2217/rme.14.77",

language = "English",

volume = "10",

pages = "135--151",

journal = "Regenerative Medicine",

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De Girolamo, L, Niada, S, Arrigoni, E, Di Giancamillo, A, Domeneghini, C, Dadsetan, M, Yaszemski, MJ, Gastaldi, D, Vena, P, Taffetani, M, Zerbi, A, Sansone, V, Peretti, GM & Brini, AT 2015, 'Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells', Regenerative Medicine, vol. 10, no. 2, pp. 135-151. https://doi.org/10.2217/rme.14.77

TY - JOUR

T1 - Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells

AU - De Girolamo, Laura

AU - Niada, Stefania

AU - Arrigoni, Elena

AU - Di Giancamillo, Alessia

AU - Domeneghini, Cinzia

AU - Dadsetan, Mahrokh

AU - Yaszemski, Michael J.

AU - Gastaldi, Dario

AU - Vena, Pasquale

AU - Taffetani, Matteo

AU - Zerbi, Alberto

AU - Sansone, Valerio

AU - Peretti, Giuseppe M.

AU - Brini, Anna T.

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Aim: Critical knee osteochondral defects in seven adult minipigs were treated with oligo(polyethylene glycol)fumarate (OPF) hydrogel combined with autologous or human adipose-derived stem cells (ASCs), and evaluated after 6 months. Methods: Four defects were made on the peripheral part of right trochleas (n = 28), and treated with OPF scaffold alone or pre-seeded with ASCs. Results: A better quality cartilage tissue characterized by improved biomechanical properties and higher collagen type II expression was observed in the defects treated by autologous or human ASC-loaded OPF; similarly this approach induced the regeneration of more mature bone with upregulation of collagen type I expression. Conclusion: This study provides the evidence that both porcine and human adipose-derived stem cells associated to OPF hydrogel allow improving osteochondral defect regeneration in a minipig model.

AB - Aim: Critical knee osteochondral defects in seven adult minipigs were treated with oligo(polyethylene glycol)fumarate (OPF) hydrogel combined with autologous or human adipose-derived stem cells (ASCs), and evaluated after 6 months. Methods: Four defects were made on the peripheral part of right trochleas (n = 28), and treated with OPF scaffold alone or pre-seeded with ASCs. Results: A better quality cartilage tissue characterized by improved biomechanical properties and higher collagen type II expression was observed in the defects treated by autologous or human ASC-loaded OPF; similarly this approach induced the regeneration of more mature bone with upregulation of collagen type I expression. Conclusion: This study provides the evidence that both porcine and human adipose-derived stem cells associated to OPF hydrogel allow improving osteochondral defect regeneration in a minipig model.

KW - adipose-derived stem cells

KW - bone

KW - cartilage

KW - oligo(polyethylene glycol)fumarate hydrogel

KW - osteochondral defects

UR - https://www.scopus.com/pages/publications/84926453443

U2 - 10.2217/rme.14.77

DO - 10.2217/rme.14.77

M3 - Article (Academic Journal)

C2 - 25835479

AN - SCOPUS:84926453443

SN - 1746-0751

VL - 10

SP - 135

EP - 151

JO - Regenerative Medicine

JF - Regenerative Medicine

IS - 2

ER -

Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells

Abstract

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Keywords

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