Resistance of ovine, caprine and bovine endothelial cells to Clostridium perfringens type D epsilon toxin in vitro

F A Uzal, B E Rolfe, N J Smith, A C Thomas, W R Kelly

Research output: Contribution to journalArticle (Academic Journal)peer-review

11 Citations (Scopus)

Abstract

Ovine, caprine and bovine endothelial cells were grown in vitro and challenged with Clostridium perfringens type D epsilon toxin to compare their susceptibility to this toxin. Madin Darby canine kidney (MDCK) cells, which are known to be susceptible to epsilon toxin, were used as a positive control. No morphological alterations were observed in any of the endothelial cell cultures tested, even after challenging with doses as high as 1200 MLD50/ml of epsilon toxin. MDCK cells showed contour rounding and nuclear condensation as early as 30 min after exposure to 100 MLD50/ml of epsilon toxin and after 60 min of exposure to 12.5 MLD50/ml of the same toxin. All the MDCK cells were dead after 3 h of exposure to all concentrations of epsilon toxin. The results indicate that ovine, caprine and bovine endothelial cells are not morphologically responsive to the action of epsilon toxin in vitro.

Original languageEnglish
Pages (from-to)275-84
Number of pages10
JournalVeterinary Research Communications
Volume23
Issue number5
Publication statusPublished - Aug 1999

Keywords

  • Animals
  • Aorta
  • Bacterial Toxins
  • Cattle
  • Cattle Diseases
  • Cells, Cultured
  • Clostridium perfringens
  • Dogs
  • Endothelium, Vascular
  • Enterotoxemia
  • Fluorescent Antibody Technique
  • Goat Diseases
  • Goats
  • Microscopy, Confocal
  • Microscopy, Phase-Contrast
  • Sheep
  • Sheep Diseases

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