Retracing in correlative light electron microscopy: Where is my object of interest?

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

6 Citations (Scopus)

Abstract

Correlative light electron microscopy (CLEM) combines the strengths of light and electron microscopy in a single experiment. There are many ways to perform a CLEM experiment and a variety of microscopy modalities can be combined either on separate instruments or as completely integrated solutions. In general, however, a CLEM experiment can be divided into three parts: probes, processing, and analysis. Most of the existing technologies are focussed around the development and use of probes or describe processing methodologies that explain or circumvent some of the compromises that need to be made when performing both light and electron microscopy on the same sample. So far, relatively little attention has been paid to the analysis part of CLEM experiments. Although it is an essential part of each CLEM experiment, it is usually a cumbersome manual process. Here, we briefly discuss each of the three above-mentioned steps, with a focus on the analysis part. We will also introduce an automated registration algorithm that can be applied to the analysis stage to enable the accurate registration of LM and EM images. This facilitates tracing back the right cell/object seen in the light microscope in the EM.

Original languageEnglish
Title of host publicationMethods in Cell Biology
Pages1-20
Number of pages20
Volume124
DOIs
Publication statusPublished - 2014

Bibliographical note

© 2014 Elsevier Inc. All rights reserved.

Keywords

  • CLEM
  • Correlative light electron microscopy
  • Electron microscopy
  • Electron tomography
  • Overlay mapping
  • Retracing

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