Rnd3-induced cell rounding requires interaction with Plexin-B2

Brad McColl, Ritu Garg, Philippe Riou, Kirsi Riento, Anne J Ridley

Research output: Contribution to journalArticle (Academic Journal)peer-review

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Abstract

Rnd proteins are atypical members of the Rho GTPase family that induce actin cytoskeletal reorganization and cell rounding. Rnd proteins have been reported to bind to the intracellular domain of several plexin receptors, but whether plexins contribute to the Rnd-induced rounding response is not known. Here we show that Rnd3 interacts preferentially with plexin-B2 of the three plexin-B proteins, whereas Rnd2 interacts with all three B-type plexins, and Rnd1 shows only very weak interaction with plexin-B proteins in immunoprecipitations. Plexin-B1 has been reported to act as a GAP for R-Ras and/or Rap1 proteins. We show that all three plexin-B proteins interact with R-Ras and Rap1, but Rnd proteins do not alter this interaction or R-Ras or Rap1 activity. We demonstrate that plexin-B2 promotes Rnd3-induced cell rounding and loss of stress fibres, and enhances the inhibition of HeLa cell invasion by Rnd3. We identify the amino acids in Rnd3 that are required for plexin-B2 interaction, and show that mutation of these amino acids prevents Rnd3-induced morphological changes. These results indicate that plexin-B2 is a downstream target for Rnd3, which contributes to its cellular function.

Original languageEnglish
Pages (from-to)4046-4056
Number of pages11
JournalJournal of Cell Science
Volume129
Issue number21
Early online date21 Sep 2016
DOIs
Publication statusPublished - 1 Nov 2016

Keywords

  • Animals
  • COS Cells
  • Cell Adhesion Molecules
  • Cell Shape
  • Cercopithecus aethiops
  • HeLa Cells
  • Humans
  • Mice
  • Nerve Tissue Proteins
  • Phosphorylation
  • Protein Binding
  • Protein Domains
  • rap GTP-Binding Proteins
  • ras Proteins
  • rho GTP-Binding Proteins
  • Journal Article
  • Research Support, Non-U.S. Gov't

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