Role of protein kinase C-ε (PKCε) in isoflurane-induced cardioprotection

D Obal, NC Weber, KD Zacharowski, O Toma, S Dettwiler, JI Wolter, M Kratz, J Müllenheim, B Preckel, W Schlack

    Research output: Contribution to journalArticle (Academic Journal)peer-review

    48 Citations (Scopus)

    Abstract

    Background. Volatile anaesthetics precondition the heart against infarction, an effect partly mediated by activation of the {varepsilon} isoform of protein kinase C (PKC{varepsilon}). We investigated whether cardioprotection by activation of PKC{varepsilon} depends on the isoflurane concentration. Methods. Anaesthetized rats underwent 25 min of coronary artery occlusion followed by 120 min of reperfusion and were randomly assigned to the following groups (n=10 in each group): isoflurane preconditioning induced by 15 min administration of 0.4 minimal alveolar concentration (MAC) (0.4MAC), 1 MAC (1MAC) or 1.75 MAC (1.75MAC) followed by 10 min washout before ischaemia. Each protocol was repeated in the presence of the PKC inhibitor staurosporine (10 µg kg–1): 0.4MAC+S, 1MAC+S and 1.75MAC+S. Controls were untreated (CON) and additional hearts received staurosporine without isoflurane (S). In a second set of experiments (n=6 in each group) hearts were excised before the infarct inducing ischaemia, and phosphorylation and translocation of PKC{varepsilon} were determined by western blot analysis. Results. Isoflurane reduced infarct size from a mean of 61(SEM 2)% of the area at risk in controls to 20(1)% (0.4MAC), 26(3)% (1MAC) and 30(1)% (1.75MAC) (all P
    Translated title of the contributionRole of protein kinase C-ε (PKCε) in isoflurane-induced cardioprotection
    Original languageEnglish
    Pages (from-to)166 - 173
    JournalBritish Journal of Anaesthesia
    Volume94 (2)
    DOIs
    Publication statusPublished - Feb 2005

    Bibliographical note

    Publisher: Oxford University Press

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