Roles of crustacean hyperglycaemic hormone in ionic and metabolic homeostasis in the Christmas Island blue crab, Discoplax celeste

Lucy M. Turner*, Simon G. Webster, Stephen Morris

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

12 Citations (Scopus)

Abstract

There is a growing body of evidence implicating the involvement of crustacean hyperglycaemic hormone (CHH) in ionic homeostasis in decapod crustaceans. However, little is known regarding hormonally influenced osmoregulatory processes in terrestrial decapods. As many terrestrial decapods experience opposing seasonal demands upon ionoregulatory physiologies, we reasoned that these would make interesting models in which to study the effect of CHH upon these phenomena. In particular, those (tropical) species that also undergo seasonal migrations might be especially informative, as we know relatively little regarding the nature of CHHs in terrestrial decapods, and hormonally mediated responses to seasonal changes in metabolic demands might also be superimposed or otherwise integrated with those associated with ionic homeostasis. Using Discoplax celeste as a model crab that experiences seasonal extremes in water availability, and exhibits diurnal and migratory activity patterns, we identified two CHHs in the sinus gland. We biochemically characterised (cDNA cloning) one CHH and functionally characterised (in terms of dose-dependent hyperglycaemic responses and glucose-dependent negative feedback loops) both CHHs. Whole-animal in situ branchial chamber (NaCl)-Na-22 perfusion experiments showed that injection of both CHHs increased gill Na+ uptake in a seasonally dependent manner, and Cr-51-EDTA clearance experiments demonstrated that CHH increased urine production by the antennal gland. Seasonal and salinity-dependent differences in haemolymph CHH titre further implicated CHH in osmoregulatory processes. Intriguingly, CHH appeared to have no effect on gill Na+/K+-ATPase or V-ATPase activity, suggesting unknown mechanisms of this hormone's action on Na+ transport across gill epithelia.

Original languageEnglish
Pages (from-to)1191-1201
Number of pages11
JournalJournal of Experimental Biology
Volume216
Issue number7
DOIs
Publication statusPublished - Apr 2013

Keywords

  • sinus gland
  • CHH
  • osmoregulation
  • season
  • Gecarcoidea natalis
  • MOLT-INHIBITING HORMONE
  • FRESH-WATER CRAB
  • ACTIVE NACL ABSORPTION
  • EURYHALINE GREEN CRAB
  • AMINO-ACID-SEQUENCE
  • ERIOCHEIR-SINENSIS
  • CHINESE CRAB
  • CARCINUS-MAENAS
  • POSTERIOR GILLS
  • TERRESTRIAL EXISTENCE

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