Rolled-up functionalized nanomembranes as three-dimensional cavities for single cell studies

Wang Xi, Christine K Schmidt, Samuel Sanchez, David H Gracias, Rafael E Carazo-Salas, Stephen P Jackson, Oliver G Schmidt

Research output: Contribution to journalArticle (Academic Journal)peer-review

66 Citations (Scopus)


We use micropatterning and strain engineering to encapsulate single living mammalian cells into transparent tubular architectures consisting of three-dimensional (3D) rolled-up nanomembranes. By using optical microscopy, we demonstrate that these structures are suitable for the scrutiny of cellular dynamics within confined 3D-microenvironments. We show that spatial confinement of mitotic mammalian cells inside tubular architectures can perturb metaphase plate formation, delay mitotic progression, and cause chromosomal instability in both a transformed and nontransformed human cell line. These findings could provide important clues into how spatial constraints dictate cellular behavior and function.

Original languageEnglish
Pages (from-to)4197-204
Number of pages8
JournalNano Letters
Issue number8
Publication statusPublished - 13 Aug 2014


  • HeLa Cells
  • Humans
  • Membranes, Artificial
  • Metaphase
  • Nanostructures
  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.


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