ROS of Distinct Sources and Salicylic Acid Separate Elevated CO2-Mediated Stomatal Movements in Arabidopsis

Jingjing He, Ruo Xi Zhang, Dae Sung Kim, Peng Sun, Honggang Liu, Zhongming Liu, Alistair M. Hetherington, Yun Kuan Liang*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

3 Citations (Scopus)

Abstract

Elevated CO2 (eCO2) often reduces leaf stomatal aperture and density thus impacts plant physiology and productivity. We have previously demonstrated that the Arabidopsis BIG protein distinguishes between the processes of eCO2-induced stomatal closure and eCO2-inhibited stomatal opening. However, the mechanistic basis of this action is not fully understood. Here we show that eCO2-elicited reactive oxygen species (ROS) production in big mutants was compromised in stomatal closure induction but not in stomatal opening inhibition. Pharmacological and genetic studies show that ROS generated by both NADPH oxidases and cell wall peroxidases contribute to eCO2-induced stomatal closure, whereas inhibition of light-induced stomatal opening by eCO2 may rely on the ROS derived from NADPH oxidases but not from cell wall peroxidases. As with JA and ABA, SA is required for eCO2-induced ROS generation and stomatal closure. In contrast, none of these three signals has a significant role in eCO2-inhibited stomatal opening, unveiling the distinct roles of plant hormonal signaling pathways in the induction of stomatal closure and the inhibition of stomatal opening by eCO2. In conclusion, this study adds SA to a list of plant hormones that together with ROS from distinct sources distinguish two branches of eCO2-mediated stomatal movements.

Original languageEnglish
Article number542
JournalFrontiers in Plant Science
Volume11
DOIs
Publication statusPublished - 8 May 2020

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China under Grant No. 31770282 and 31470360 and the UK, BBSRC, Grant No. BB/N001168/1.

Publisher Copyright:
© Copyright © 2020 He, Zhang, Kim, Sun, Liu, Liu, Hetherington and Liang.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Keywords

  • cell wall peroxidases
  • elevated CO
  • NADPH oxidases
  • plant hormones
  • reactive oxygen species
  • stomatal movement

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