S-adenosyl homocysteine and DNA ends stimulate promiscuous nuclease activities in the Type III restriction endonuclease EcoPI

LJ Peakman, MD Szczelkun

Research output: Contribution to journalArticle (Academic Journal)

9 Citations (Scopus)

Abstract

In the absence of the methyl donor S-adenosyl methionine and under certain permissive reaction conditions, EcoPI shows non-specific endonuclease activity. We show here that the cofactor analogue S-adenosyl homocysteine promotes this promiscuous DNA cleavage. Additionally, an extensive exonuclease-like processing of the DNA is also observed that can even result in digestion of non-specific DNA in trans. We suggest a model for how DNA communication events initiating from non-specific sites, and in particular free DNA ends, could produce the observed cleavage patterns.
Translated title of the contributionS-adenosyl homocysteine and DNA ends stimulate promiscuous nuclease activities in the Type III restriction endonuclease EcoPI
Original languageEnglish
Pages (from-to)3934 - 3945
Number of pages12
JournalNucleic Acids Research
Volume37 (12)
DOIs
Publication statusPublished - Jul 2009

Bibliographical note

Other: First published online 28th April 2009

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