Scanning confocal near-infra-red microscopy: a new microscopy technique for three-dimensional histopathology

MSN Murthy; MG Jones; J Kulka; Jack D Davies; M Halliwell; PC Jackson; David Bull; PNT Wells

doi:10.1049/esej:19950509

Scanning confocal near-infra-red microscopy: a new microscopy technique for three-dimensional histopathology

MSN Murthy, MG Jones, J Kulka, Jack D Davies, M Halliwell, PC Jackson, David Bull, PNT Wells

Research output: Contribution to journal › Article (Academic Journal) › peer-review

Abstract

To image microstructures within tissue in detail using conventional light microscopy it is necessary to process the tissue and obtain thin (5 μm) physical sections by using destructive physical sectioning techniques. This article describes a microscopy technique, using near-infra-red light (700-1500 nm) as the source, which is able to image thin sections of thick-tissue specimens nondestructively. Images of contiguous two-dimensional thin sections obtained serially can be combined to generate a three-dimensional image of the structures being imaged.

Original language	English
Pages (from-to)	223 - 230
Journal	Engineering Science and Education Journal
Volume	4
Issue number	5
DOIs	https://doi.org/10.1049/esej:19950509
Publication status	Published - Oct 1995

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Publisher: Institution of Electrical Engineers (IEE)

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http://hdl.handle.net/1983/1438

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title = "Scanning confocal near-infra-red microscopy: a new microscopy technique for three-dimensional histopathology",

abstract = "To image microstructures within tissue in detail using conventional light microscopy it is necessary to process the tissue and obtain thin (5 μm) physical sections by using destructive physical sectioning techniques. This article describes a microscopy technique, using near-infra-red light (700-1500 nm) as the source, which is able to image thin sections of thick-tissue specimens nondestructively. Images of contiguous two-dimensional thin sections obtained serially can be combined to generate a three-dimensional image of the structures being imaged.",

author = "MSN Murthy and MG Jones and J Kulka and Davies, {Jack D} and M Halliwell and PC Jackson and David Bull and PNT Wells",

note = "Publisher: Institution of Electrical Engineers (IEE)",

year = "1995",

month = oct,

doi = "10.1049/esej:19950509",

language = "English",

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pages = "223 -- 230",

journal = "Engineering Science and Education Journal",

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T1 - Scanning confocal near-infra-red microscopy: a new microscopy technique for three-dimensional histopathology

AU - Murthy, MSN

AU - Jones, MG

AU - Kulka, J

AU - Davies, Jack D

AU - Halliwell, M

AU - Jackson, PC

AU - Bull, David

AU - Wells, PNT

N1 - Publisher: Institution of Electrical Engineers (IEE)

PY - 1995/10

Y1 - 1995/10

N2 - To image microstructures within tissue in detail using conventional light microscopy it is necessary to process the tissue and obtain thin (5 μm) physical sections by using destructive physical sectioning techniques. This article describes a microscopy technique, using near-infra-red light (700-1500 nm) as the source, which is able to image thin sections of thick-tissue specimens nondestructively. Images of contiguous two-dimensional thin sections obtained serially can be combined to generate a three-dimensional image of the structures being imaged.

AB - To image microstructures within tissue in detail using conventional light microscopy it is necessary to process the tissue and obtain thin (5 μm) physical sections by using destructive physical sectioning techniques. This article describes a microscopy technique, using near-infra-red light (700-1500 nm) as the source, which is able to image thin sections of thick-tissue specimens nondestructively. Images of contiguous two-dimensional thin sections obtained serially can be combined to generate a three-dimensional image of the structures being imaged.

U2 - 10.1049/esej:19950509

DO - 10.1049/esej:19950509

M3 - Article (Academic Journal)

SN - 0963-7346

VL - 4

SP - 223

EP - 230

JO - Engineering Science and Education Journal

JF - Engineering Science and Education Journal

IS - 5

ER -

Scanning confocal near-infra-red microscopy: a new microscopy technique for three-dimensional histopathology

Abstract

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