To image microstructures within tissue in detail using conventional light microscopy it is necessary to process the tissue and obtain thin (5 μm) physical sections by using destructive physical sectioning techniques. This article describes a microscopy technique, using near-infra-red light (700-1500 nm) as the source, which is able to image thin sections of thick-tissue specimens nondestructively. Images of contiguous two-dimensional thin sections obtained serially can be combined to generate a three-dimensional image of the structures being imaged.
Bibliographical notePublisher: Institution of Electrical Engineers (IEE)
Murthy, MSN., Jones, MG., Kulka, J., Davies, J. D., Halliwell, M., Jackson, PC., Bull, D., & Wells, PNT. (1995). Scanning confocal near-infra-red microscopy: a new microscopy technique for three-dimensional histopathology. Engineering Science and Education Journal, 4(5), 223 - 230. https://doi.org/10.1049/esej:19950509