Segmentation and analysis of insulin granule membranes in beta islet cell electron micrographs

David C Nam; Judith Mantell; David R Bull; Paul Verkade; Alin Achim

Segmentation and analysis of insulin granule membranes in beta islet cell electron micrographs

David C Nam, Judith Mantell, David R Bull, Paul Verkade, Alin Achim

Research output: Chapter in Book/Report/Conference proceeding › Conference Contribution (Conference Proceeding)

3 Citations (Scopus)

Abstract

Quantification of sub cellular structures is necessary in understanding how cells function. This paper presents a segmentation algorithm for transmission electron microscopy images of insulin granule membranes from beta cells of rat islet of Langerhans. Granules are described as having a dense core and a surrounding halo. We use a mixed vector field convolution snake to segment the granule membranes. We also present a novel contribution to the convergence filter family, which uses an adjustable region of support. The filter is used to verify our segmentation. We calculate pixel error by comparing the membrane areas from our method with a manually defined ground truth. 1300 granules are used in our test and an average area difference of 7.54% is observed.

Original language	English
Title of host publication	2012 Proceedings of the 20th European Signal Processing Conference (EUSIPCO 2012)
Publisher	Institute of Electrical and Electronics Engineers (IEEE)
Pages	2228-2232
Number of pages	5
ISBN (Print)	9781467310680
Publication status	Published - Jan 2013
Event	20th European Signal Processing Conference (EUSIPCO) - Bucharest, Romania Duration: 27 Aug 2012 → 31 Aug 2012

Publication series

Name	Proceedings of the European Signal Processing Conference (EUSIPCO)
Publisher	Institute of Electrical and Electronics Engineers (IEEE)
ISSN (Print)	2076-1465

Conference

Conference	20th European Signal Processing Conference (EUSIPCO)
Country	Romania
City	Bucharest
Period	27/08/12 → 31/08/12

Access to Document

http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=6333790

Cite this

Nam, D. C., Mantell, J., Bull, D. R., Verkade, P., & Achim, A. (2013). Segmentation and analysis of insulin granule membranes in beta islet cell electron micrographs. In 2012 Proceedings of the 20th European Signal Processing Conference (EUSIPCO 2012) (pp. 2228-2232). (Proceedings of the European Signal Processing Conference (EUSIPCO)). Institute of Electrical and Electronics Engineers (IEEE). http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=6333790

Nam, David C ; Mantell, Judith ; Bull, David R ; Verkade, Paul ; Achim, Alin. / Segmentation and analysis of insulin granule membranes in beta islet cell electron micrographs. 2012 Proceedings of the 20th European Signal Processing Conference (EUSIPCO 2012) . Institute of Electrical and Electronics Engineers (IEEE), 2013. pp. 2228-2232 (Proceedings of the European Signal Processing Conference (EUSIPCO)).

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title = "Segmentation and analysis of insulin granule membranes in beta islet cell electron micrographs",

abstract = "Quantification of sub cellular structures is necessary in understanding how cells function. This paper presents a segmentation algorithm for transmission electron microscopy images of insulin granule membranes from beta cells of rat islet of Langerhans. Granules are described as having a dense core and a surrounding halo. We use a mixed vector field convolution snake to segment the granule membranes. We also present a novel contribution to the convergence filter family, which uses an adjustable region of support. The filter is used to verify our segmentation. We calculate pixel error by comparing the membrane areas from our method with a manually defined ground truth. 1300 granules are used in our test and an average area difference of 7.54% is observed.",

author = "Nam, {David C} and Judith Mantell and Bull, {David R} and Paul Verkade and Alin Achim",

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series = "Proceedings of the European Signal Processing Conference (EUSIPCO)",

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note = "20th European Signal Processing Conference (EUSIPCO) ; Conference date: 27-08-2012 Through 31-08-2012",

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Nam, DC, Mantell, J , Bull, DR , Verkade, P & Achim, A 2013, Segmentation and analysis of insulin granule membranes in beta islet cell electron micrographs. in 2012 Proceedings of the 20th European Signal Processing Conference (EUSIPCO 2012) . Proceedings of the European Signal Processing Conference (EUSIPCO), Institute of Electrical and Electronics Engineers (IEEE), pp. 2228-2232, 20th European Signal Processing Conference (EUSIPCO), Bucharest, Romania, 27/08/12. <http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=6333790>

Segmentation and analysis of insulin granule membranes in beta islet cell electron micrographs. / Nam, David C; Mantell, Judith ; Bull, David R ; Verkade, Paul ; Achim, Alin.

2012 Proceedings of the 20th European Signal Processing Conference (EUSIPCO 2012) . Institute of Electrical and Electronics Engineers (IEEE), 2013. p. 2228-2232 (Proceedings of the European Signal Processing Conference (EUSIPCO)).

Research output: Chapter in Book/Report/Conference proceeding › Conference Contribution (Conference Proceeding)

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T1 - Segmentation and analysis of insulin granule membranes in beta islet cell electron micrographs

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AU - Mantell, Judith

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AU - Verkade, Paul

AU - Achim, Alin

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AB - Quantification of sub cellular structures is necessary in understanding how cells function. This paper presents a segmentation algorithm for transmission electron microscopy images of insulin granule membranes from beta cells of rat islet of Langerhans. Granules are described as having a dense core and a surrounding halo. We use a mixed vector field convolution snake to segment the granule membranes. We also present a novel contribution to the convergence filter family, which uses an adjustable region of support. The filter is used to verify our segmentation. We calculate pixel error by comparing the membrane areas from our method with a manually defined ground truth. 1300 granules are used in our test and an average area difference of 7.54% is observed.

M3 - Conference Contribution (Conference Proceeding)

SN - 9781467310680

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ER -

Nam DC, Mantell J , Bull DR , Verkade P , Achim A. Segmentation and analysis of insulin granule membranes in beta islet cell electron micrographs. In 2012 Proceedings of the 20th European Signal Processing Conference (EUSIPCO 2012) . Institute of Electrical and Electronics Engineers (IEEE). 2013. p. 2228-2232. (Proceedings of the European Signal Processing Conference (EUSIPCO)).