Self-transformation and structural reconfiguration in coacervate-based protocells

Stephen Mann; Avinash Patil; Robert Harniman; Ravinash Krishna Kumar

doi:10.1039/C6SC00205F

Self-transformation and structural reconfiguration in coacervate-based protocells

Stephen Mann, Avinash Patil, Robert Harniman, Ravinash Krishna Kumar

Research output: Contribution to journal › Article (Academic Journal) › peer-review

31 Citations (Scopus)

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Abstract

A functionalized small-molecule dipeptide capable of structural adaptation is used to prepare coacervatebased protocells that exhibit a pH-triggered process of self-transformation and structural reconfiguration. Polymer-dipeptide coacervate micro-droplets are prepared at pH 8.5 from aqueous mixtures of
poly(diallyldimethylammonium chloride) and deprotonated N-(fluorenyl-9-methoxycarbonyl)-D-Ala-D-Ala, and transform into discrete aster-like micro-architectures by controlled lowering of the pH to 4.5. Reconfiguration of the micro-droplets results in entanglement and formation of an interpenetrating
fibrous network that subsequently develops into a polymer-containing dipeptide hydrogel. Our results provide a step towards the assembly of synthetic protocells exhibiting rudimentary aspects of metamorphosis, and should offer a new approach to the design and construction of soft reconfigurable chemical micro-ensembles.

Original language	English
Pages (from-to)	5879-5887
Number of pages	9
Journal	Chemical Science
Volume	7
Issue number	9
Early online date	25 May 2016
DOIs	https://doi.org/10.1039/C6SC00205F
Publication status	Published - 1 Sept 2016

Keywords

Coacervate droplets
Protocells
Self-assembled fibers
Hydrogels
Soft-Matter

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This is the final published version of the article (version of record). It first appeared online via RSC at http://pubs.rsc.org/en/Content/ArticleLanding/2016/SC/C6SC00205F#!divAbstract. Please refer to any applicable terms of use of the publisher.
Final published version, 2.03 MBLicence: CC BY

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title = "Self-transformation and structural reconfiguration in coacervate-based protocells",

abstract = "A functionalized small-molecule dipeptide capable of structural adaptation is used to prepare coacervatebased protocells that exhibit a pH-triggered process of self-transformation and structural reconfiguration. Polymer-dipeptide coacervate micro-droplets are prepared at pH 8.5 from aqueous mixtures ofpoly(diallyldimethylammonium chloride) and deprotonated N-(fluorenyl-9-methoxycarbonyl)-D-Ala-D-Ala, and transform into discrete aster-like micro-architectures by controlled lowering of the pH to 4.5. Reconfiguration of the micro-droplets results in entanglement and formation of an interpenetratingfibrous network that subsequently develops into a polymer-containing dipeptide hydrogel. Our results provide a step towards the assembly of synthetic protocells exhibiting rudimentary aspects of metamorphosis, and should offer a new approach to the design and construction of soft reconfigurable chemical micro-ensembles.",

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author = "Stephen Mann and Avinash Patil and Robert Harniman and {Krishna Kumar}, Ravinash",

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AU - Mann, Stephen

AU - Patil, Avinash

AU - Harniman, Robert

AU - Krishna Kumar, Ravinash

PY - 2016/9/1

Y1 - 2016/9/1

N2 - A functionalized small-molecule dipeptide capable of structural adaptation is used to prepare coacervatebased protocells that exhibit a pH-triggered process of self-transformation and structural reconfiguration. Polymer-dipeptide coacervate micro-droplets are prepared at pH 8.5 from aqueous mixtures ofpoly(diallyldimethylammonium chloride) and deprotonated N-(fluorenyl-9-methoxycarbonyl)-D-Ala-D-Ala, and transform into discrete aster-like micro-architectures by controlled lowering of the pH to 4.5. Reconfiguration of the micro-droplets results in entanglement and formation of an interpenetratingfibrous network that subsequently develops into a polymer-containing dipeptide hydrogel. Our results provide a step towards the assembly of synthetic protocells exhibiting rudimentary aspects of metamorphosis, and should offer a new approach to the design and construction of soft reconfigurable chemical micro-ensembles.

AB - A functionalized small-molecule dipeptide capable of structural adaptation is used to prepare coacervatebased protocells that exhibit a pH-triggered process of self-transformation and structural reconfiguration. Polymer-dipeptide coacervate micro-droplets are prepared at pH 8.5 from aqueous mixtures ofpoly(diallyldimethylammonium chloride) and deprotonated N-(fluorenyl-9-methoxycarbonyl)-D-Ala-D-Ala, and transform into discrete aster-like micro-architectures by controlled lowering of the pH to 4.5. Reconfiguration of the micro-droplets results in entanglement and formation of an interpenetratingfibrous network that subsequently develops into a polymer-containing dipeptide hydrogel. Our results provide a step towards the assembly of synthetic protocells exhibiting rudimentary aspects of metamorphosis, and should offer a new approach to the design and construction of soft reconfigurable chemical micro-ensembles.

KW - Coacervate droplets

KW - Protocells

KW - Self-assembled fibers

KW - Hydrogels

KW - Soft-Matter

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U2 - 10.1039/C6SC00205F

DO - 10.1039/C6SC00205F

M3 - Article (Academic Journal)

SN - 2041-6520

VL - 7

SP - 5879

EP - 5887

JO - Chemical Science

JF - Chemical Science

IS - 9

ER -

Self-transformation and structural reconfiguration in coacervate-based protocells

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Self-transformation and structural reconfiguration in coacervate-based protocells

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