Sequential acquisition of T-cells and antibodies to nontyphoidal Salmonella in Malawian Children

Tonney S Nyirenda; James J Gilchrist; Nicholas A Feasey; Sarah J Glennie; Naor Bar-Zeev; Melita A Gordon; Calman A Maclennan; Wilson L Mandala; Robert S Heyderman

doi:10.1093/infdis/jiu045

Sequential acquisition of T-cells and antibodies to nontyphoidal Salmonella in Malawian Children

Tonney S Nyirenda, James J Gilchrist, Nicholas A Feasey, Sarah J Glennie, Naor Bar-Zeev, Melita A Gordon, Calman A Maclennan, Wilson L Mandala, Robert S Heyderman

School of Cellular and Molecular Medicine

Research output: Contribution to journal › Article (Academic Journal) › peer-review

44 Citations (Scopus)

Abstract

Background. S. Typhimurium (STm) remain a prominent cause of bacteremia in sub-Saharan Africa. Complement-fixing antibodies to STm develop by 2 years of age. We hypothesized that STm-specific CD4+ T cells develop alongside this process.Methods. Eighty healthy Malawian children aged 0-60 months were recruited. STm-specific CD4+ T cells producing IFNγ, TNFα and IL2 were quantified using intra-cellular cytokine staining. Antibodies to STm were measured by serum bactericidal activity assay (SBA), and anti-STm IgG antibodies by ELISA.Results. Between 2006 and 2011, 449 STm bacteremias were detected in children

Original language	English
Journal	Journal of Infectious Diseases
DOIs	https://doi.org/10.1093/infdis/jiu045
Publication status	Published - 16 Jan 2014

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@article{3da4722f5da94d429560ec6fe13e31af,

title = "Sequential acquisition of T-cells and antibodies to nontyphoidal Salmonella in Malawian Children",

abstract = "Background. S. Typhimurium (STm) remain a prominent cause of bacteremia in sub-Saharan Africa. Complement-fixing antibodies to STm develop by 2 years of age. We hypothesized that STm-specific CD4+ T cells develop alongside this process.Methods. Eighty healthy Malawian children aged 0-60 months were recruited. STm-specific CD4+ T cells producing IFNγ, TNFα and IL2 were quantified using intra-cellular cytokine staining. Antibodies to STm were measured by serum bactericidal activity assay (SBA), and anti-STm IgG antibodies by ELISA.Results. Between 2006 and 2011, 449 STm bacteremias were detected in children ",

author = "Nyirenda, {Tonney S} and Gilchrist, {James J} and Feasey, {Nicholas A} and Glennie, {Sarah J} and Naor Bar-Zeev and Gordon, {Melita A} and Maclennan, {Calman A} and Mandala, {Wilson L} and Heyderman, {Robert S}",

year = "2014",

month = jan,

day = "16",

doi = "10.1093/infdis/jiu045",

language = "English",

journal = "Journal of Infectious Diseases",

issn = "1537-6613",

publisher = "Oxford University Press",

}

TY - JOUR

T1 - Sequential acquisition of T-cells and antibodies to nontyphoidal Salmonella in Malawian Children

AU - Nyirenda, Tonney S

AU - Gilchrist, James J

AU - Feasey, Nicholas A

AU - Glennie, Sarah J

AU - Bar-Zeev, Naor

AU - Gordon, Melita A

AU - Maclennan, Calman A

AU - Mandala, Wilson L

AU - Heyderman, Robert S

PY - 2014/1/16

Y1 - 2014/1/16

N2 - Background. S. Typhimurium (STm) remain a prominent cause of bacteremia in sub-Saharan Africa. Complement-fixing antibodies to STm develop by 2 years of age. We hypothesized that STm-specific CD4+ T cells develop alongside this process.Methods. Eighty healthy Malawian children aged 0-60 months were recruited. STm-specific CD4+ T cells producing IFNγ, TNFα and IL2 were quantified using intra-cellular cytokine staining. Antibodies to STm were measured by serum bactericidal activity assay (SBA), and anti-STm IgG antibodies by ELISA.Results. Between 2006 and 2011, 449 STm bacteremias were detected in children

AB - Background. S. Typhimurium (STm) remain a prominent cause of bacteremia in sub-Saharan Africa. Complement-fixing antibodies to STm develop by 2 years of age. We hypothesized that STm-specific CD4+ T cells develop alongside this process.Methods. Eighty healthy Malawian children aged 0-60 months were recruited. STm-specific CD4+ T cells producing IFNγ, TNFα and IL2 were quantified using intra-cellular cytokine staining. Antibodies to STm were measured by serum bactericidal activity assay (SBA), and anti-STm IgG antibodies by ELISA.Results. Between 2006 and 2011, 449 STm bacteremias were detected in children

U2 - 10.1093/infdis/jiu045

DO - 10.1093/infdis/jiu045

M3 - Article (Academic Journal)

C2 - 24443544

SN - 1537-6613

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

ER -

Sequential acquisition of T-cells and antibodies to nontyphoidal Salmonella in Malawian Children

Abstract

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