Abstract
Introduction: Serotonin (5-HT) signaling in the heart is mediated by receptor subtype 2B (5-HTR2B). While contribution of serotonin to canine valvular disease is reported, the role of 5-HTR2B role in other canine cardiac diseases is not yet known.
Material and Methods: Blood samples of each nine healthy control dogs and dogs with congestive heart failure (CHF), and myocardial samples of eight healthy control dogs, nine dogs with cardiac diseases and six dogs with systemic non-cardiac diseases were investigated for 5-HTR2B transcription by quantitative PCR (qPCR). Myocardial results were correlated with the transcription of cytokines, matrix metalloproteinases (MMP) and tissue inhibitors of matrix metalloproteinase (TIMP). To identify cells producing 5-HTR2B, laser microdissection with subsequent qPCR and immunohistology were applied.
Results: Control dogs exhibited constitutive 5-HTR2B transcription in blood and myocardium. Dogs with CHF showed significantly higher circulating 5-HTR2B levels than control dogs, while myocardial 5-HTR2B transcription was significantly highest in dogs with dilated cardiomyopathy (DCM) compared to all other groups, and a positive correlation of 5-HTR2B with several cytokines, MMPs and TIMPs was observed. Myocytes were identified as the source of 5-HTR2B mRNA and protein.
Conclusions: The present study provides evidence that serotonin plays a role in normal cardiac structure and function and is involved in CHF and the pathogenesis of DCM.
Material and Methods: Blood samples of each nine healthy control dogs and dogs with congestive heart failure (CHF), and myocardial samples of eight healthy control dogs, nine dogs with cardiac diseases and six dogs with systemic non-cardiac diseases were investigated for 5-HTR2B transcription by quantitative PCR (qPCR). Myocardial results were correlated with the transcription of cytokines, matrix metalloproteinases (MMP) and tissue inhibitors of matrix metalloproteinase (TIMP). To identify cells producing 5-HTR2B, laser microdissection with subsequent qPCR and immunohistology were applied.
Results: Control dogs exhibited constitutive 5-HTR2B transcription in blood and myocardium. Dogs with CHF showed significantly higher circulating 5-HTR2B levels than control dogs, while myocardial 5-HTR2B transcription was significantly highest in dogs with dilated cardiomyopathy (DCM) compared to all other groups, and a positive correlation of 5-HTR2B with several cytokines, MMPs and TIMPs was observed. Myocytes were identified as the source of 5-HTR2B mRNA and protein.
Conclusions: The present study provides evidence that serotonin plays a role in normal cardiac structure and function and is involved in CHF and the pathogenesis of DCM.
| Original language | English |
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| Publication status | Published - 6 Sept 2013 |
| Event | European Society and College of Veterinary Pathologists - London, United Kingdom Duration: 4 Sept 2013 → 7 Sept 2013 |
Conference
| Conference | European Society and College of Veterinary Pathologists |
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| Country/Territory | United Kingdom |
| City | London |
| Period | 4/09/13 → 7/09/13 |