SNARES in opposing bilayers interact in a circular array to form conducting pores

SJ Cho, M Kelly, KT Rognlien, JA Cho, JKH Hoerber, BP Jena

Research output: Contribution to journalArticle (Academic Journal)peer-review

97 Citations (Scopus)

Abstract

The process of fusion at the nerve terminal is mediated via a specialized set of proteins in the synaptic vesicles and the presynaptic membrane. Three soluble N-ethylmaleimide-sensitive factor (NSF)-attachment protein receptors (SNAREs) have been implicated in membrane fusion. The structure and arrangement of these SNAREs associated with lipid bilayers were examined using atomic force microscopy. A bilayer electrophysiological setup allowed for measurements of membrane conductance and capacitance. Here we demonstrate that the interaction of these proteins to form a fusion pore is dependent on the presence of t-SNAREs and v-SNARE in opposing bilayers. Addition of purified recombinant v-SNARE to a t-SNARE-reconstituted lipid membrane increased only the size of the globular t-SNARE oligomer without influencing the electrical properties of the membrane. However when t-SNARE vesicles were added to a v-SNARE membrane, SNAREs assembles in a ring pattern and a stepwise increase in capacitance, and increase in conductance were observed. Thus, t- and v-SNAREs are required to reside in opposing bilayers to allow appropriate t-/v-SNARE interactions leading to membrane fusion.
Translated title of the contributionSNARES in opposing bilayers interact in a circular array to form conducting pores
Original languageEnglish
Pages (from-to)2522 - 2527
Number of pages6
JournalBiophysical Journal
Volume83(5)
Publication statusPublished - 2002

Bibliographical note

Publisher: Biophysical Society, USA

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