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SNX15 links clathrin endocytosis to the PtdIns(3)P early endosome independent of the APPL1 endosome

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SNX15 links clathrin endocytosis to the PtdIns(3)P early endosome independent of the APPL1 endosome. / Danson, Chris; Brown, Edward; Hemmings, Oliver J; McGough, Ian J; Yarwood, Sam; Heesom, Kate J; Carlton, Jeremy G; Martin-Serrano, Juan; May, Margaret T; Verkade, Paul; Cullen, Peter J.

In: Journal of Cell Science, Vol. 126, 28.08.2013, p. 4885-4899.

Research output: Contribution to journalArticle

Harvard

Danson, C, Brown, E, Hemmings, OJ, McGough, IJ, Yarwood, S, Heesom, KJ, Carlton, JG, Martin-Serrano, J, May, MT, Verkade, P & Cullen, PJ 2013, 'SNX15 links clathrin endocytosis to the PtdIns(3)P early endosome independent of the APPL1 endosome', Journal of Cell Science, vol. 126, pp. 4885-4899. https://doi.org/10.1242/jcs.125732

APA

Vancouver

Danson C, Brown E, Hemmings OJ, McGough IJ, Yarwood S, Heesom KJ et al. SNX15 links clathrin endocytosis to the PtdIns(3)P early endosome independent of the APPL1 endosome. Journal of Cell Science. 2013 Aug 28;126:4885-4899. https://doi.org/10.1242/jcs.125732

Author

Danson, Chris ; Brown, Edward ; Hemmings, Oliver J ; McGough, Ian J ; Yarwood, Sam ; Heesom, Kate J ; Carlton, Jeremy G ; Martin-Serrano, Juan ; May, Margaret T ; Verkade, Paul ; Cullen, Peter J. / SNX15 links clathrin endocytosis to the PtdIns(3)P early endosome independent of the APPL1 endosome. In: Journal of Cell Science. 2013 ; Vol. 126. pp. 4885-4899.

Bibtex

@article{8646866313da4aa1a11e1bda4677dc4f,
title = "SNX15 links clathrin endocytosis to the PtdIns(3)P early endosome independent of the APPL1 endosome",
abstract = "Sorting nexins (SNXs) are key regulators of the endosomal network. In designing an RNAi-mediated loss-of-function screen, we establish that of thirty human SNXs only SNX3, SNX5, SNX9, SNX15 and SNX21 appear to regulate EGF receptor degradative sorting. Suppression of SNX15 results in a delay in receptor degradation arising from a defect in movement of newly internalised EGF receptor-labelled vesicles into early endosomes. Besides a PtdIns(3)P- and PX domain-dependent association to early endosomes, SNX15 also associates with clathrin-coated pits and clathrin-coated vesicles via direct binding to clathrin through a non-canonical clathrin-binding box. From live cell imaging, the activated EGF receptor enters distinct sub-populations of SNX15- and APPL1-labelled peripheral endocytic vesicles, which do not undergo heterotypic fusion. The SNX15-decorated receptor-containing sub-population does however undergo direct fusion with the Rab5-labelled early endosome. Our data is consistent with a model in which the EGF receptor enters the early endosome following clathrin-mediated endocytosis through at least two parallel pathways: maturation via an APPL1-intermediate compartment and an alternative more direct fusion between SNX15 decorated endocytic vesicles and the Rab5-positive early endosome.",
author = "Chris Danson and Edward Brown and Hemmings, {Oliver J} and McGough, {Ian J} and Sam Yarwood and Heesom, {Kate J} and Carlton, {Jeremy G} and Juan Martin-Serrano and May, {Margaret T} and Paul Verkade and Cullen, {Peter J}",
year = "2013",
month = "8",
day = "28",
doi = "10.1242/jcs.125732",
language = "English",
volume = "126",
pages = "4885--4899",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",

}

RIS - suitable for import to EndNote

TY - JOUR

T1 - SNX15 links clathrin endocytosis to the PtdIns(3)P early endosome independent of the APPL1 endosome

AU - Danson, Chris

AU - Brown, Edward

AU - Hemmings, Oliver J

AU - McGough, Ian J

AU - Yarwood, Sam

AU - Heesom, Kate J

AU - Carlton, Jeremy G

AU - Martin-Serrano, Juan

AU - May, Margaret T

AU - Verkade, Paul

AU - Cullen, Peter J

PY - 2013/8/28

Y1 - 2013/8/28

N2 - Sorting nexins (SNXs) are key regulators of the endosomal network. In designing an RNAi-mediated loss-of-function screen, we establish that of thirty human SNXs only SNX3, SNX5, SNX9, SNX15 and SNX21 appear to regulate EGF receptor degradative sorting. Suppression of SNX15 results in a delay in receptor degradation arising from a defect in movement of newly internalised EGF receptor-labelled vesicles into early endosomes. Besides a PtdIns(3)P- and PX domain-dependent association to early endosomes, SNX15 also associates with clathrin-coated pits and clathrin-coated vesicles via direct binding to clathrin through a non-canonical clathrin-binding box. From live cell imaging, the activated EGF receptor enters distinct sub-populations of SNX15- and APPL1-labelled peripheral endocytic vesicles, which do not undergo heterotypic fusion. The SNX15-decorated receptor-containing sub-population does however undergo direct fusion with the Rab5-labelled early endosome. Our data is consistent with a model in which the EGF receptor enters the early endosome following clathrin-mediated endocytosis through at least two parallel pathways: maturation via an APPL1-intermediate compartment and an alternative more direct fusion between SNX15 decorated endocytic vesicles and the Rab5-positive early endosome.

AB - Sorting nexins (SNXs) are key regulators of the endosomal network. In designing an RNAi-mediated loss-of-function screen, we establish that of thirty human SNXs only SNX3, SNX5, SNX9, SNX15 and SNX21 appear to regulate EGF receptor degradative sorting. Suppression of SNX15 results in a delay in receptor degradation arising from a defect in movement of newly internalised EGF receptor-labelled vesicles into early endosomes. Besides a PtdIns(3)P- and PX domain-dependent association to early endosomes, SNX15 also associates with clathrin-coated pits and clathrin-coated vesicles via direct binding to clathrin through a non-canonical clathrin-binding box. From live cell imaging, the activated EGF receptor enters distinct sub-populations of SNX15- and APPL1-labelled peripheral endocytic vesicles, which do not undergo heterotypic fusion. The SNX15-decorated receptor-containing sub-population does however undergo direct fusion with the Rab5-labelled early endosome. Our data is consistent with a model in which the EGF receptor enters the early endosome following clathrin-mediated endocytosis through at least two parallel pathways: maturation via an APPL1-intermediate compartment and an alternative more direct fusion between SNX15 decorated endocytic vesicles and the Rab5-positive early endosome.

U2 - 10.1242/jcs.125732

DO - 10.1242/jcs.125732

M3 - Article

C2 - 23986476

VL - 126

SP - 4885

EP - 4899

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

ER -