Abstract
In recent years, Correlative Multimodal Imaging (CMI) has become an "en vogue" technique and a bit of a buzzword. It entails combining information from different imaging modalities to extract more information from a sample that would otherwise not be possible from each individual technique. The best established CMI technology is correlative light and electron microscopy (CLEM), which applies light and electron microscopy on the exact same sample/structure. In general, it entails the detection of fluorescently tagged proteins or structures by light microscopy and subsequently their relative intracellular localization is determined with nanometer resolution using transmission electron microscopy (TEM). Here, we describe the different steps involved in a "simple" CLEM approach. We describe the overall workflow, instrumentation, and basic principles of sample preparation for a CLEM experiment exploiting stable expression of fluorescent proteins.
Original language | English |
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Title of host publication | Imaging Cell Signalling |
Publisher | Humana Press |
Pages | 89-102 |
Number of pages | 14 |
ISBN (Electronic) | 9781071638347 |
ISBN (Print) | 9781071638330 |
DOIs | |
Publication status | E-pub ahead of print - 7 May 2024 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 2800 |
ISSN (Print) | 1064-3745 |
ISSN (Electronic) | 1940-6029 |
Bibliographical note
Publisher Copyright:© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.
Keywords
- Humans
- Microscopy, Electron, Transmission/methods
- Microscopy, Fluorescence/methods
- Microscopy, Electron/methods
- Image Processing, Computer-Assisted/methods
- Animals