Spatial localization and projection densities of brainstem mossy fibre afferents to the forelimb C1 zone of the rat cerebellum

Luis Herrero, Joanne Pardoe, Nadia L Cerminara, Richard Apps

Research output: Contribution to journalArticle (Academic Journal)peer-review

5 Citations (Scopus)

Abstract

The present study uses a double retrograde tracer technique in rats to examine the spatial localization and pattern of axonal branching in mossy fibres arising from three major sources in the medulla-the external cuneate nucleus, the sensory trigeminal nucleus and the reticular formation, to two electrophysiologically-identified parts of the cerebellar cortex that are linked by common climbing fibre input - the forelimb-receiving parts of the C1 zone in lobulus simplex and the paramedian lobule. In each experiment a small injection of rhodamine-tagged beads was injected into one cortical region and an injection of fluorescein-tagged beads was injected into the other region. The main findings were: (i) the proportion of double-labelled cells in each of the three precerebeller sources of mossy fibres was positively correlated with those in the inferior olive; and (ii) the C1 zone in lobulus simplex was found to receive a greater density of projections from all three sources of mossy fibres than the C1 zone in the paramedian lobule. These data suggest that two rostrocaudally separated but somatotopically corresponding parts of the C1 zone receive common mossy fibre and climbing fibre inputs. However, the differences in projection densities also suggest that the two parts of the zone differ in the extent to which they receive mossy fibre signals arising from the same precerebellar nuclei. This implies differences in function between somatotopically corresponding parts of the same cortical zone, and could enable a higher degree of parallel processing and integration of information within them.
Original languageEnglish
Pages (from-to)539-49
Number of pages11
JournalEuropean Journal of Neuroscience
Volume35
Issue number4
DOIs
Publication statusPublished - Feb 2012

Bibliographical note

© 2012 The Authors. European Journal of Neuroscience © 2012 Federation of European Neuroscience Societies and Blackwell Publishing Ltd.

Keywords

  • Animals
  • Cell Count
  • Forelimb
  • Cerebellum
  • Fluorescein
  • Electric Stimulation
  • Rats
  • Brain Mapping
  • Neurons
  • Rats, Wistar
  • Brain Stem
  • Rhodamines
  • Afferent Pathways
  • Time Factors
  • Functional Laterality
  • Male
  • Nerve Fibers

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