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Spatiotemporal quantification of acoustic cell patterning using Voronoï tessellation

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)562-573
Number of pages12
JournalLab on a Chip
Volume19
Issue number4
Early online date22 Jan 2019
DOIs
DateAccepted/In press - 30 Nov 2018
DateE-pub ahead of print - 22 Jan 2019
DatePublished (current) - 12 Feb 2019

Abstract

Acoustic patterning using ultrasound standing waves has recently emerged as a potent biotechnology enabling the remote generation of ordered cell systems. This capability has opened up exciting opportunities, for example, in guiding the development of organoid cultures or the organization of complex tissues. The success of these studies is often contingent on the formation of tightly-packed and uniform cell arrays; however, a number of factors can act to disrupt or prevent acoustic patterning. Yet, to the best of our knowledge, there has been no comprehensive assessment of the quality of acoustically-patterned cell populations. In this report we use a mathematical approach, known as Voronoï tessellation, to generate a series of metrics that can be used to measure the effect of cell concentration, pressure amplitude, ultrasound frequency and biomaterial viscosity upon the quality of acoustically-patterned cell systems. Moreover, we extend this approach towards the characterization of spatiotemporal processes, namely, the acoustic patterning of cell suspensions and the migration of patterned, adherent cell clusters. This strategy is simple, unbiased and highly informative, and we anticipate that the methods described here will provide a systematic framework for all stages of acoustic patterning, including the robust quality control of devices, statistical comparison of patterning conditions, the quantitative exploration of parameter limits and the ability to track patterned tissue formation over time.

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via Royal Society of Chemistry at https://doi.org/10.1039/c8lc01108g . Please refer to any applicable terms of use of the publisher.

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via Royal Society of Chemistry at https://doi.org/10.1039/c8lc01108g . Please refer to any applicable terms of use of the publisher.

    Final published version, 3 MB, PDF document

    Licence: CC BY

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