Species differences in the morphology of T-tubule openings in cardiomyocytes

Eva Rog-Zielinska, Cherrie H T Kong, C M Zgierski-Johnston, Paul Verkade, Judith Mantell, Mark Cannell, Peter Kohl

Research output: Contribution to journalArticle (Academic Journal)

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Abstract

Aims: The ultrastructure of ventricular cardiomyocyte T-tubule connections with the outer cell surface has been reported to be different between mice and rabbits. In mice, T-tubules form convoluted narrow spaces filled with electron-dense matter that impedes diffusion between T-tubular lumen and bulk extracellular space. Here, we explore whether T-tubule mouths are also restricted in rat (another murine model used frequently for cardiac research) and whether pig and human T-tubule mouths are also generally restricted as is the case for mouse.

Methods: We used chemically-fixed tissue and high-pressure frozen cells to compare T-tubule mouth architecture in rats, mice, and rabbits using transmission electron microscopy and 3D electron tomography.

Results: We find that rat T-tubular mouth architecture is more similar to that of rabbits than mice, lacking the marked tortuosity and electron-dense ground substance that obstructs access to deeper portions of the T-tubular system in mice. Pilot observations in larger mammals (pig, human) suggest that mouse may be the least representative animal model of T-tubule connectivity with the outer cell surface in larger mammals.

Conclusion: Rat T-tubular system architecture appears to be more similar in size and topology to other mammals than mice. T-tubular mouth topology may contribute to differences in experimental model behaviour, underscoring the problems of appropriate model selection for research into cell and tissue functions.
Original languageEnglish
Pages (from-to)iii120-iii124
Number of pages5
JournalEP-Europace
Volume20
Issue numberSupp 3
DOIs
Publication statusPublished - 1 Nov 2018

Keywords

  • Transverse tubules
  • heart
  • cardiac
  • ultrastructure
  • electron tomography

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    Rog-Zielinska, E., Kong, C. H. T., Zgierski-Johnston, C. M., Verkade, P., Mantell, J., Cannell, M., & Kohl, P. (2018). Species differences in the morphology of T-tubule openings in cardiomyocytes. EP-Europace, 20(Supp 3), iii120-iii124. https://doi.org/10.1093/europace/euy245