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Species differences in the morphology of T-tubule openings in cardiomyocytes

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Species differences in the morphology of T-tubule openings in cardiomyocytes. / Rog-Zielinska, Eva; Kong, Cherrie H T; Zgierski-Johnston, C M; Verkade, Paul; Mantell, Judith; Cannell, Mark; Kohl, Peter.

In: EP-Europace, Vol. 20, No. Supp 3, 01.11.2018, p. iii120-iii124.

Research output: Contribution to journalArticle

Harvard

Rog-Zielinska, E, Kong, CHT, Zgierski-Johnston, CM, Verkade, P, Mantell, J, Cannell, M & Kohl, P 2018, 'Species differences in the morphology of T-tubule openings in cardiomyocytes', EP-Europace, vol. 20, no. Supp 3, pp. iii120-iii124. https://doi.org/10.1093/europace/euy245

APA

Rog-Zielinska, E., Kong, C. H. T., Zgierski-Johnston, C. M., Verkade, P., Mantell, J., Cannell, M., & Kohl, P. (2018). Species differences in the morphology of T-tubule openings in cardiomyocytes. EP-Europace, 20(Supp 3), iii120-iii124. https://doi.org/10.1093/europace/euy245

Vancouver

Rog-Zielinska E, Kong CHT, Zgierski-Johnston CM, Verkade P, Mantell J, Cannell M et al. Species differences in the morphology of T-tubule openings in cardiomyocytes. EP-Europace. 2018 Nov 1;20(Supp 3):iii120-iii124. https://doi.org/10.1093/europace/euy245

Author

Rog-Zielinska, Eva ; Kong, Cherrie H T ; Zgierski-Johnston, C M ; Verkade, Paul ; Mantell, Judith ; Cannell, Mark ; Kohl, Peter. / Species differences in the morphology of T-tubule openings in cardiomyocytes. In: EP-Europace. 2018 ; Vol. 20, No. Supp 3. pp. iii120-iii124.

Bibtex

@article{f277744f103544ab969fca4a6729e6c2,
title = "Species differences in the morphology of T-tubule openings in cardiomyocytes",
abstract = "Aims: The ultrastructure of ventricular cardiomyocyte T-tubule connections with the outer cell surface has been reported to be different between mice and rabbits. In mice, T-tubules form convoluted narrow spaces filled with electron-dense matter that impedes diffusion between T-tubular lumen and bulk extracellular space. Here, we explore whether T-tubule mouths are also restricted in rat (another murine model used frequently for cardiac research) and whether pig and human T-tubule mouths are also generally restricted as is the case for mouse. Methods: We used chemically-fixed tissue and high-pressure frozen cells to compare T-tubule mouth architecture in rats, mice, and rabbits using transmission electron microscopy and 3D electron tomography. Results: We find that rat T-tubular mouth architecture is more similar to that of rabbits than mice, lacking the marked tortuosity and electron-dense ground substance that obstructs access to deeper portions of the T-tubular system in mice. Pilot observations in larger mammals (pig, human) suggest that mouse may be the least representative animal model of T-tubule connectivity with the outer cell surface in larger mammals.Conclusion: Rat T-tubular system architecture appears to be more similar in size and topology to other mammals than mice. T-tubular mouth topology may contribute to differences in experimental model behaviour, underscoring the problems of appropriate model selection for research into cell and tissue functions.",
keywords = "Transverse tubules, heart, cardiac, ultrastructure, electron tomography",
author = "Eva Rog-Zielinska and Kong, {Cherrie H T} and Zgierski-Johnston, {C M} and Paul Verkade and Judith Mantell and Mark Cannell and Peter Kohl",
year = "2018",
month = "11",
day = "1",
doi = "10.1093/europace/euy245",
language = "English",
volume = "20",
pages = "iii120--iii124",
journal = "EP-Europace",
issn = "1099-5129",
publisher = "Oxford University Press",
number = "Supp 3",

}

RIS - suitable for import to EndNote

TY - JOUR

T1 - Species differences in the morphology of T-tubule openings in cardiomyocytes

AU - Rog-Zielinska, Eva

AU - Kong, Cherrie H T

AU - Zgierski-Johnston, C M

AU - Verkade, Paul

AU - Mantell, Judith

AU - Cannell, Mark

AU - Kohl, Peter

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Aims: The ultrastructure of ventricular cardiomyocyte T-tubule connections with the outer cell surface has been reported to be different between mice and rabbits. In mice, T-tubules form convoluted narrow spaces filled with electron-dense matter that impedes diffusion between T-tubular lumen and bulk extracellular space. Here, we explore whether T-tubule mouths are also restricted in rat (another murine model used frequently for cardiac research) and whether pig and human T-tubule mouths are also generally restricted as is the case for mouse. Methods: We used chemically-fixed tissue and high-pressure frozen cells to compare T-tubule mouth architecture in rats, mice, and rabbits using transmission electron microscopy and 3D electron tomography. Results: We find that rat T-tubular mouth architecture is more similar to that of rabbits than mice, lacking the marked tortuosity and electron-dense ground substance that obstructs access to deeper portions of the T-tubular system in mice. Pilot observations in larger mammals (pig, human) suggest that mouse may be the least representative animal model of T-tubule connectivity with the outer cell surface in larger mammals.Conclusion: Rat T-tubular system architecture appears to be more similar in size and topology to other mammals than mice. T-tubular mouth topology may contribute to differences in experimental model behaviour, underscoring the problems of appropriate model selection for research into cell and tissue functions.

AB - Aims: The ultrastructure of ventricular cardiomyocyte T-tubule connections with the outer cell surface has been reported to be different between mice and rabbits. In mice, T-tubules form convoluted narrow spaces filled with electron-dense matter that impedes diffusion between T-tubular lumen and bulk extracellular space. Here, we explore whether T-tubule mouths are also restricted in rat (another murine model used frequently for cardiac research) and whether pig and human T-tubule mouths are also generally restricted as is the case for mouse. Methods: We used chemically-fixed tissue and high-pressure frozen cells to compare T-tubule mouth architecture in rats, mice, and rabbits using transmission electron microscopy and 3D electron tomography. Results: We find that rat T-tubular mouth architecture is more similar to that of rabbits than mice, lacking the marked tortuosity and electron-dense ground substance that obstructs access to deeper portions of the T-tubular system in mice. Pilot observations in larger mammals (pig, human) suggest that mouse may be the least representative animal model of T-tubule connectivity with the outer cell surface in larger mammals.Conclusion: Rat T-tubular system architecture appears to be more similar in size and topology to other mammals than mice. T-tubular mouth topology may contribute to differences in experimental model behaviour, underscoring the problems of appropriate model selection for research into cell and tissue functions.

KW - Transverse tubules

KW - heart

KW - cardiac

KW - ultrastructure

KW - electron tomography

U2 - 10.1093/europace/euy245

DO - 10.1093/europace/euy245

M3 - Article

VL - 20

SP - iii120-iii124

JO - EP-Europace

JF - EP-Europace

SN - 1099-5129

IS - Supp 3

ER -