Abstract
Recent work has revealed that, contrary to expectations, the protein machineries responsible for regulating cellular polarity in fission yeast cells localises cortically to the semi-spherical tips of each cell as punctual structures. To quantify this discovery and try and reconcile it with previous findings, a statistical co-localisation framework is set up. We use spatial statistics to analyse collections of punctual objects detected from deconvolved wide-field fluorescence microscopy images as point patterns from their respective spatial point processes. The theory is described for spherical data, as the structures of interest reside on a half-sphere in 3D. Uses include test of complete independence with Monte-Carlo hypothesis testing and comparison between pairs of point collections using bootstrap hypothesis testing. Evaluation on simulated data are performed on top of the targeted biological studies.
Original language | English |
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Title of host publication | 2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012 - Proceedings |
Pages | 1747-1750 |
Number of pages | 4 |
DOIs | |
Publication status | Published - 2012 |
Event | 2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012 - Barcelona, Spain Duration: 2 May 2012 → 5 May 2012 |
Conference
Conference | 2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012 |
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Country/Territory | Spain |
City | Barcelona |
Period | 2/05/12 → 5/05/12 |
Keywords
- Cell biology
- Cell Polarity
- Co-localisation
- Fission yeast
- Fluorescence microscopy
- Schizosaccharomyces pombe
- Spatial Statistics