Spherical spatial statistics for 3D fluorescence video-microscopy

A. Chessel*, J. Dodgson, R. E. Carazo-Salas

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference Contribution (Conference Proceeding)

2 Citations (Scopus)

Abstract

Recent work has revealed that, contrary to expectations, the protein machineries responsible for regulating cellular polarity in fission yeast cells localises cortically to the semi-spherical tips of each cell as punctual structures. To quantify this discovery and try and reconcile it with previous findings, a statistical co-localisation framework is set up. We use spatial statistics to analyse collections of punctual objects detected from deconvolved wide-field fluorescence microscopy images as point patterns from their respective spatial point processes. The theory is described for spherical data, as the structures of interest reside on a half-sphere in 3D. Uses include test of complete independence with Monte-Carlo hypothesis testing and comparison between pairs of point collections using bootstrap hypothesis testing. Evaluation on simulated data are performed on top of the targeted biological studies.

Original languageEnglish
Title of host publication2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012 - Proceedings
Pages1747-1750
Number of pages4
DOIs
Publication statusPublished - 2012
Event2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012 - Barcelona, Spain
Duration: 2 May 20125 May 2012

Conference

Conference2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012
CountrySpain
CityBarcelona
Period2/05/125/05/12

Keywords

  • Cell biology
  • Cell Polarity
  • Co-localisation
  • Fission yeast
  • Fluorescence microscopy
  • Schizosaccharomyces pombe
  • Spatial Statistics

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    Chessel, A., Dodgson, J., & Carazo-Salas, R. E. (2012). Spherical spatial statistics for 3D fluorescence video-microscopy. In 2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012 - Proceedings (pp. 1747-1750). [6235918] https://doi.org/10.1109/ISBI.2012.6235918