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Abstract
Molecularly crowded, polyelectrolyte/ribonucleotide-enriched membrane-free coacervate droplets are transformed into membrane-bounded sub-divided vesicles by using a polyoxometalate-mediated surface-templating procedure. The coacervate to vesicle transition results in reconstruction of the coacervate micro-droplets into novel three-tiered micro-compartments comprising a semi-permeable negatively charged polyoxometalate/polyelectrolyte outer membrane, a sub-membrane coacervate shell, and an internal aqueous lumen. We demonstrate that organic dyes, ssDNA, magnetic nanoparticles and enzymes can be concentrated into the interior of the micro-compartments by sequestration into the coacervate micro-droplets prior to vesicle formation. The vesicle-encapsulated proteins are inaccessible to proteases in the external medium, and can be exploited for the spatial localization and coupling of two-enzyme cascade reactions within single or between multiple populations of hybrid vesicles dispersed in aqueous media.
Original language | English |
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Pages (from-to) | 1830-1840 |
Number of pages | 11 |
Journal | Small |
Volume | 10 |
Issue number | 9 |
DOIs | |
Publication status | Published - May 2014 |
Keywords
- GENE-EXPRESSION
- LIPID VESICLES
- CAPSULES
- POLYMER
- ENZYMES
- CELLS
- REACTIVITY
- PARTICLES
- DROPLETS
- RELEASE
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