Spontaneous Structuration in Coacervate-Based Protocells by Polyoxometalate-Mediated Membrane Assembly

David S. Williams, Avinash J. Patil, Stephen Mann*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

43 Citations (Scopus)

Abstract

Molecularly crowded, polyelectrolyte/ribonucleotide-enriched membrane-free coacervate droplets are transformed into membrane-bounded sub-divided vesicles by using a polyoxometalate-mediated surface-templating procedure. The coacervate to vesicle transition results in reconstruction of the coacervate micro-droplets into novel three-tiered micro-compartments comprising a semi-permeable negatively charged polyoxometalate/polyelectrolyte outer membrane, a sub-membrane coacervate shell, and an internal aqueous lumen. We demonstrate that organic dyes, ssDNA, magnetic nanoparticles and enzymes can be concentrated into the interior of the micro-compartments by sequestration into the coacervate micro-droplets prior to vesicle formation. The vesicle-encapsulated proteins are inaccessible to proteases in the external medium, and can be exploited for the spatial localization and coupling of two-enzyme cascade reactions within single or between multiple populations of hybrid vesicles dispersed in aqueous media.

Original languageEnglish
Pages (from-to)1830-1840
Number of pages11
JournalSmall
Volume10
Issue number9
DOIs
Publication statusPublished - May 2014

Keywords

  • GENE-EXPRESSION
  • LIPID VESICLES
  • CAPSULES
  • POLYMER
  • ENZYMES
  • CELLS
  • REACTIVITY
  • PARTICLES
  • DROPLETS
  • RELEASE

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