Strain typing to highlight particular points of interest in the control of Staphylococcus aureus mastitis

D. G. J. Prins*, A. Smith, D. C. Barrett, K. A. Ellis

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

Abstract

As an aid to evaluate an existing Staphylococcus aureus control programme implemented on an organic dairy farm, staphylococci isolates from bovine teat skin, milking gloves, milking equipment and bovine milk were identified to species level using the Staph ID 32 API system. Phenotypically and biochemically identified S. aureus isolates were analysed by pulsed-field gel electrophoresis (PFGE) and DNA sequence analysis of the X region of the protein A gene (Spa). PFGE identified two main S. aureus types with two subtypes each. Spa typing also identified two main types; one of which consisted of two subtypes. The results were used to identify possible transmission pathways of S. aureus strains and to address potential points of weakness in the S. aureus mastitis control programme.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalCattle Practice
Volume21
Publication statusPublished - Apr 2013

Keywords

  • Staphylococcus aureus
  • mastitis
  • pulse-field gel electrophoresis
  • spa typing
  • intramammary infection
  • FIELD GEL-ELECTROPHORESIS
  • MOLECULAR EPIDEMIOLOGIC ANALYSIS
  • INTRAMAMMARY INFECTIONS
  • EXTRAMAMMARY SITES
  • PROTEIN-A
  • BOVINE
  • COWS
  • PHAGE
  • PATTERNS
  • HERDS

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