Strobilurin biosynthesis in Basidiomycete fungi

Risa Nofiani, Kate de Mattos-Shipley, Karen E. Lebe, Li Chen Han, Zafar Iqbal, Andrew M. Bailey, Christine L. Willis, Thomas J. Simpson, Russell J. Cox*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

15 Citations (Scopus)
273 Downloads (Pure)

Abstract

Strobilurins from fungi are the inspiration for the creation of the β-methoxyacrylate class of agricultural fungicides. However, molecular details of the biosynthesis of strobilurins have remained cryptic. Here we report the sequence of genomes of two fungi that produce strobilurins and show that each contains a biosynthetic gene cluster, which encodes a highly reducing polyketide synthase with very unusual C-terminal hydrolase and methyltransferase domains. Expression of stpks1 in Aspergillus oryzae leads to the production of prestrobilurin A when the fermentation is supplemented with a benzoyl coenzyme A (CoA) analogue. This enables the discovery of a previously unobserved route to benzoyl CoA. Reconstruction of the gene cluster in A. oryzae leads to the formation of prestrobilurin A, and addition of the gene str9 encoding an FAD-dependent oxygenase leads to the key oxidative rearrangement responsible for the creation of the β-methoxyacrylate toxophore. Finally, two methyltransferases are required to complete the synthesis.

Original languageEnglish
Article number3940
Number of pages11
JournalNature Communications
Volume9
DOIs
Publication statusPublished - 26 Sep 2018

Structured keywords

  • BrisSynBio
  • Bristol BioDesign Institute

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