Structural elucidation and environmental distributions of butanetriol and pentanetriol dialkyl glycerol tetraethers (BDGTs and PDGTs)

Sarah Coffinet*, Travis B. Meador, Lukas Mühlena, Kevin W. Becker, Jan Schröder, Qing Zeng Zhu, Julius S. Lipp, Verena B. Heuer, Matthew P. Crump, Kai Uwe Hinrichs

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

4 Citations (Scopus)
39 Downloads (Pure)

Abstract

Butanetriol and pentanetriol dialkyl glycerol tetraethers (BDGTs and PDGTs) are membrane lipids, recently discovered in sedimentary environments and in the methanogenic archaeon Methanomassiliicoccus luminyensis. They possess an unusual structure, which challenges fundamental assumptions in lipid biochemistry. Indeed, they bear a butanetriol or a pentanetriol backbone instead of a glycerol at one end of their core structure. In this study, we unambiguously located the additional methyl group of the BDGT compound on the C3 carbon of the lipid backbone via high-field nuclear magnetic resonance (NMR) experiments. We further systematically explored the abundance, distribution and isotopic composition of BDGTs and PDGTs as both intact polar and core lipid forms in marine sediments collected in contrasting environments of the Mediterranean Sea and Black Sea. High proportions of intact polar BDGTs and PDGTs in the deeper methane-laden sedimentary layers and relatively 13C-depleted BDGTs, especially in the Rhone Delta and in the Black Sea, are in agreement with a probable methanogenic source for these lipids. However, contributions from heterotrophic Archaea to BDGTs (and PDGTs) cannot be excluded, particularly in the eastern Mediterranean Sea, and contrasting BDGT and PDGT headgroup distribution patterns were observed between the different sites studied. This points to additional, non-methanogenic, archaeal sources for these lipids.

Original languageEnglish
Pages (from-to)317-330
Number of pages14
JournalBiogeosciences
Volume17
Issue number2
DOIs
Publication statusPublished - 22 Jan 2020

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