Structure and sequence of the gene encoding the alpha-subunit of rat translation initiation factor-2B

K M Flowers, H Mellor, S R Kimball, L S Jefferson

    Research output: Contribution to journalArticle (Academic Journal)peer-review

    Abstract

    Eukaryotic initiation factor-2B (eIF-2B) is a heteropentameric guanine nucleotide exchange factor involved in the recycling of eIF-2 during the translation initiation phase of protein synthesis. The activity of eIF-2B is controlled by diverse mechanisms under a wide range of conditions. As part of our studies into the control of the expression of eIF-2B subunit mRNAs, we have isolated and characterized the rat gene encoding the alpha-subunit of eIF-2B. The gene is comprised of 9 exons which are contained within 8.5 kb of genomic DNA. A comparison of the 5'-flanking region of the gene with that of the previously isolated eIF-2B epsilon gene reveals that both possess a potential binding site for the alpha-Pal transcription factor as well as several other regions of homology.

    Original languageEnglish
    Pages (from-to)163-7
    Number of pages5
    JournalBiochimica et Biophysica Acta (BBA)
    Volume1264
    Issue number2
    DOIs
    Publication statusPublished - 7 Nov 1995

    Keywords

    • Animals
    • Base Sequence
    • Brain/metabolism
    • Consensus Sequence
    • DNA Primers
    • DNA, Complementary
    • Guanine Nucleotide Exchange Factors
    • Introns
    • Macromolecular Substances
    • Molecular Sequence Data
    • Protein Biosynthesis
    • Proteins/genetics
    • Rats/genetics
    • Restriction Mapping

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