Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats

HL Winton, RD Lund, P Adamson, Y Sauve, DJ Keegan, SV Girman, S Wang, N Kanuga, ASL Kwan, L Beauchene, A Zerbib, L Hetherington, PO Couraud, P Coffey, J Greenwood

Research output: Contribution to journalArticle (Academic Journal)peer-review

169 Citations (Scopus)

Abstract

Royal College of Surgeons rats are genetically predisposed to undergo significant visual loss caused by a primary dysfunction of retinal pigment epithelial (RPE) cells. By using this model, we have examined the efficacy of subretinal transplantation of two independent human RIPE cell lines each exhibiting genetic modifications that confer long-term stability in vitro. The two cell lines, a spontaneously derived cell line (ARPE19) and an extensively characterized genetically engineered human RIPE cell line (h1RPE7), which expresses SV40 large T (tumor) antigen, were evaluated separately. Both lines result in a significant preservation of visual function as assessed by either behavioral or physiological techniques. This attenuation of visual loss correlates with photoreceptor survival and the presence of donor cells in the areas of rescued photoreceptors at 5 months postgrafting (6 months of age). These results demonstrate the potential of genetically modified human RPE cells for ultimate application in therapeutic transplantation strategies for retinal degenerative diseases caused by RIPE dysfunction.
Translated title of the contributionSubretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats
Original languageEnglish
Pages (from-to)9942 - 9947
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number17
DOIs
Publication statusPublished - 14 Aug 2001

Keywords

  • Retinal Pigment Epithelium
  • RCS Rat
  • Macular Degeneration
  • Superior colliculus
  • grafts
  • rescue

Fingerprint Dive into the research topics of 'Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats'. Together they form a unique fingerprint.

Cite this