Substrate promiscuity of a de novo designed peroxidase

Jonathan M X Jenkins, Claire E M Noble, Katie J Grayson, Adrian J Mulholland, J L Ross Anderson

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

The design and construction of de novo enzymes offer potentially facile routes to exploiting powerful chemistries in robust, expressible and customisable protein frameworks, while providing insight into natural enzyme function. To this end, we have recently demonstrated extensive catalytic promiscuity in a heme-containing de novo protein, C45. The diverse transformations that C45 catalyses include substrate oxidation, dehalogenation and carbon‑carbon bond formation. Here we explore the substrate promiscuity of C45's peroxidase activity, screening the de novo enzyme against a panel of peroxidase and dehaloperoxidase substrates. Consistent with the function of natural peroxidases, C45 exhibits a broad spectrum of substrate activities with selectivity dictated primarily by the redox potential of the substrate, and by extension, the active oxidising species in peroxidase chemistry, compounds I and II. Though the comparison of these redox potentials provides a threshold for determining activity for a given substrate, substrate:protein interactions are also likely to play a significant role in determining electron transfer rates from substrate to heme, affecting the kinetic parameters of the enzyme. We also used biomolecular simulation to screen substrates against a computational model of C45 to identify potential interactions and binding sites. Several sites of interest in close proximity to the heme cofactor were discovered, providing insight into the catalytic workings of C45.
Original languageEnglish
Article number111370
Number of pages11
JournalJournal of Inorganic Biochemistry
Volume217
Early online date12 Feb 2021
DOIs
Publication statusPublished - 1 Apr 2021

Structured keywords

  • BrisSynBio
  • Bristol BioDesign Institute

Keywords

  • peroxidases
  • de novo proteins
  • heme
  • redox enzymology
  • biomolecular simulation

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