Helicases are a ubiquitous and abundant group of motor proteins that couple NTP binding and hydrolysis to processive unwinding of nucleic acids. By targeting this activity to a wide range of specific substrates, and by coupling it with other catalytic functionality, helicases fulfil diverse roles in virtually all aspects of nucleic acid metabolism. The present review takes a look back at our efforts to elucidate the molecular mechanisms of UvrD-like DNA helicases. Using these well-studied enzymes as examples, we also discuss how helicases are programmed by interactions with partner proteins to participate in specific cellular functions.
|Translated title of the contribution||Superfamily I helicase as modular components of DNA-processing machines|
|Pages (from-to)||413 - 423|
|Number of pages||11|
|Journal||Biochemical Society Transactions|
|Publication status||Published - Apr 2011|