Surface biotinylation of primary neurons to monitor changes in AMPA receptor surface expression in response to kainate receptor stimulation

Jithin D. Nair; Jeremy M. Henley; Kevin A. Wilkinson

doi:10.1016/j.xpro.2021.100992

Surface biotinylation of primary neurons to monitor changes in AMPA receptor surface expression in response to kainate receptor stimulation

Jithin D. Nair
, Jeremy M. Henley
, Kevin A. Wilkinson

Research output: Contribution to journal › Protocol

9 Citations (Scopus)

386 Downloads (Pure)

Original language	English
Article number	100992
Journal	STAR Protocols
Volume	2
Issue number	4
Early online date	7 Dec 2021
DOIs	https://doi.org/10.1016/j.xpro.2021.100992
Publication status	Published - 17 Dec 2021

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Funding Information:
We are grateful to the BBSRC ( BB/R00787X/1 ), MRC ( MR/L003791/1 ), Leverhulme Trust ( RPG-2019-191 ), and Wellcome Trust ( 105384/Z/14/A ) for financial support. The graphical abstract and figures in this article were made using Biorender.com .

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© 2021 The Authors

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@article{04449c2be50d4eea87da29f212b039a2,

title = "Surface biotinylation of primary neurons to monitor changes in AMPA receptor surface expression in response to kainate receptor stimulation",

author = "Nair, \{Jithin D.\} and Henley, \{Jeremy M.\} and Wilkinson, \{Kevin A.\}",

note = "Funding Information: We are grateful to the BBSRC ( BB/R00787X/1 ), MRC ( MR/L003791/1 ), Leverhulme Trust ( RPG-2019-191 ), and Wellcome Trust ( 105384/Z/14/A ) for financial support. The graphical abstract and figures in this article were made using Biorender.com . Publisher Copyright: {\textcopyright} 2021 The Authors",

year = "2021",

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doi = "10.1016/j.xpro.2021.100992",

language = "English",

volume = "2",

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T1 - Surface biotinylation of primary neurons to monitor changes in AMPA receptor surface expression in response to kainate receptor stimulation

AU - Nair, Jithin D.

AU - Henley, Jeremy M.

AU - Wilkinson, Kevin A.

N1 - Funding Information: We are grateful to the BBSRC ( BB/R00787X/1 ), MRC ( MR/L003791/1 ), Leverhulme Trust ( RPG-2019-191 ), and Wellcome Trust ( 105384/Z/14/A ) for financial support. The graphical abstract and figures in this article were made using Biorender.com . Publisher Copyright: © 2021 The Authors

PY - 2021/12/17

Y1 - 2021/12/17

UR - https://doi.org/10.1016/j.xpro.2021.100992

U2 - 10.1016/j.xpro.2021.100992

DO - 10.1016/j.xpro.2021.100992

M3 - Protocol

C2 - 34934960

SN - 2666-1667

VL - 2

JO - STAR Protocols

JF - STAR Protocols

IS - 4

M1 - 100992

ER -

Surface biotinylation of primary neurons to monitor changes in AMPA receptor surface expression in response to kainate receptor stimulation

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