Surface Integrity governs the proteome of hypomineralised MJ

JE Mangum, FA Crombie, NM Kilpatrick, DJ Manton, MJ Hubbard

Research output: Contribution to journalArticle (Academic Journal)peer-review

72 Citations (Scopus)


Growing interest in the treatment and prevention of Molar/Incisor Hypomineralization (MIH) warrants investigation into the protein composition of hypomineralized enamel. Hypothesizing abnormality akin to amelogenesis imperfecta, we profiled proteins in hypomineralized enamel from human permanent first molars using a biochemical approach. Hypomineralized enamel was found to have from 3- to 15-fold higher protein content than normal, but a near-normal level of residual amelogenins. This distinguished MIH from hypomaturation defects with high residual amelogenins (amelogenesis imperfecta, fluorosis) and so typified it as a hypocalcification defect. Second, hypomineralized enamel was found to have accumulated various proteins from oral fluid and blood, with differential incorporation depending on integrity of the enamel surface. Pathogenically, these results point to a pre-eruptive disturbance of mineralization involving albumin and, in cases with post-eruptive breakdown, subsequent protein adsorption on the exposed hydroxyapatite matrix. These insights into the pathoge
Translated title of the contributionSurface Integrity governs the proteome of hypomineralised MJ
Original languageEnglish
Pages (from-to)1160 - 1165
Number of pages6
JournalJournal of Dental Research
Publication statusPublished - Oct 2010

Bibliographical note

Author of Publication Reviewed: Mangum JE, Crombie FA, Kilpatrick NM, Manton DJ, Hubbard MJ


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