Synbiotic Musa acuminata skin extract and Streptococcus salivarius K12 inhibit candida species biofilm formation

Nurul Alia Risma Rismayuddin, Puteri Elysa Alia Mohd Badri, Ahmad Faisal Ismail, Noratikah Othman, H M H N Bandara, Mohd Hafiz Arzmi*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

1 Citation (Scopus)
34 Downloads (Pure)

Abstract

This study aimed to determine the effect of synbiotic Musa acuminata skin extract (MASE) and Streptococcus salivarius K12 (K12) on Candida species biofilm formation. Liquid chromatography quadrupole time-of-flight (LC-Q-TOF-MS) was conducted to characterize MASE. To determine the effect of synbiotic on Candida biofilm, 200 µL of RPMI-1640 containing Candida, K12, and MASE were pipetted into the same well and incubated at 37 °C for 72 h. A similar protocol was repeated with K12 or MASE to determine the probiotic and prebiotic effects, respectively. Dimorphism, biofilm biomass, and Candida total cell count (TCC) were determined. A total of 60 compounds were detected in MASE. C. albicans (ALT5) and Candida lusitaniae exhibited the highest reduction in biofilm biomass when co-cultured with prebiotic (77.70 ± 7.67%) and synbiotic (97.73 ± 0.28%), respectively. All Candida spp. had decreased TCC and hyphae when co-cultured with synbiotic. In conclusion, MASE and K12 inhibit Candida biofilm formation.
Original languageEnglish
Pages (from-to)614-627
Number of pages14
JournalBiofouling
Volume38
Issue number6
DOIs
Publication statusPublished - 28 Jul 2022

Bibliographical note

Funding Information:
We would like to acknowledge the Ministry of Higher Education, Malaysia, for the funding (PRGS/1/2020/SKK08/UIAM/02/1 We would like to acknowledge the International Islamic University Malaysia (IIUM) for the laboratories, and Melbourne Dental School, The University of Melbourne for the microbial samples.

Publisher Copyright:
© 2022 Informa UK Limited, trading as Taylor & Francis Group.

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