Abstract
This study aimed to determine the effect of synbiotic Musa acuminata skin extract (MASE) and Streptococcus salivarius K12 (K12) on Candida species biofilm formation. Liquid chromatography quadrupole time-of-flight (LC-Q-TOF-MS) was conducted to characterize MASE. To determine the effect of synbiotic on Candida biofilm, 200 µL of RPMI-1640 containing Candida, K12, and MASE were pipetted into the same well and incubated at 37 °C for 72 h. A similar protocol was repeated with K12 or MASE to determine the probiotic and prebiotic effects, respectively. Dimorphism, biofilm biomass, and Candida total cell count (TCC) were determined. A total of 60 compounds were detected in MASE. C. albicans (ALT5) and Candida lusitaniae exhibited the highest reduction in biofilm biomass when co-cultured with prebiotic (77.70 ± 7.67%) and synbiotic (97.73 ± 0.28%), respectively. All Candida spp. had decreased TCC and hyphae when co-cultured with synbiotic. In conclusion, MASE and K12 inhibit Candida biofilm formation.
Original language | English |
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Pages (from-to) | 614-627 |
Number of pages | 14 |
Journal | Biofouling |
Volume | 38 |
Issue number | 6 |
DOIs | |
Publication status | Published - 28 Jul 2022 |
Bibliographical note
Funding Information:We would like to acknowledge the Ministry of Higher Education, Malaysia, for the funding (PRGS/1/2020/SKK08/UIAM/02/1 We would like to acknowledge the International Islamic University Malaysia (IIUM) for the laboratories, and Melbourne Dental School, The University of Melbourne for the microbial samples.
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