Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display

Charles Vragniau, Joshua C. Bufton, Frédéric Garzoni, Emilie Stermann, Fruzsina Rabi, Céline Terrat, Mélanie Guidetti, Véronique Josserand, Matt Williams, Christopher J. Woods, Gerardo Viedma, Phil Bates, Bernard Verrier, Laurence Chaperot, Christiane Schaffitzel, Imre Berger, Pascal Fender*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

2 Citations (Scopus)
148 Downloads (Pure)

Abstract

Self-assembling virus-like particles represent highly attractive tools for developing next-generation vaccines and protein therapeutics. We created ADDomer, an adenovirus-derived multimeric protein-based self-assembling nanoparticle scaffold engineered to facilitate plug-and-play display of multiple immunogenic epitopes from pathogens. We used cryo–electron microscopy at near-atomic resolution and implemented novel, cost-effective, high-performance cloud computing to reveal architectural features in unprecedented detail. We analyzed ADDomer interaction with components of the immune system and developed a promising first-in-kind ADDomer-based vaccine candidate to combat emerging Chikungunya infectious disease, exemplifying the potential of our approach.

Original languageEnglish
Article numbereaaw2853
Number of pages9
JournalScience Advances
Volume5
Issue number9
DOIs
Publication statusPublished - 25 Sep 2019

Structured keywords

  • Bristol BioDesign Institute
  • BrisSynBio

Keywords

  • SYNTHETIC BIOLOGY
  • minimal biology
  • Biodesign
  • Vaccine
  • advanced computing

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    Vragniau, C., Bufton, J. C., Garzoni, F., Stermann, E., Rabi, F., Terrat, C., Guidetti, M., Josserand, V., Williams, M., Woods, C. J., Viedma, G., Bates, P., Verrier, B., Chaperot, L., Schaffitzel, C., Berger, I., & Fender, P. (2019). Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display. Science Advances, 5(9), [eaaw2853]. https://doi.org/10.1126/sciadv.aaw2853