Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display

Charles Vragniau; Joshua C. Bufton; Frédéric Garzoni; Emilie Stermann; Fruzsina Rabi; Céline Terrat; Mélanie Guidetti; Véronique Josserand; Matt Williams; Christopher J. Woods; Gerardo Viedma; Phil Bates; Bernard Verrier; Laurence Chaperot; Christiane Schaffitzel; Imre Berger; Pascal Fender

doi:10.1126/sciadv.aaw2853

Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display

Charles Vragniau, Joshua C. Bufton, Frédéric Garzoni, Emilie Stermann, Fruzsina Rabi, Céline Terrat, Mélanie Guidetti, Véronique Josserand, Matt Williams, Christopher J. Woods, Gerardo Viedma, Phil Bates, Bernard Verrier, Laurence Chaperot, Christiane Schaffitzel, Imre Berger, Pascal Fender^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

28 Citations (Scopus)

280 Downloads (Pure)

Abstract

Self-assembling virus-like particles represent highly attractive tools for developing next-generation vaccines and protein therapeutics. We created ADDomer, an adenovirus-derived multimeric protein-based self-assembling nanoparticle scaffold engineered to facilitate plug-and-play display of multiple immunogenic epitopes from pathogens. We used cryo–electron microscopy at near-atomic resolution and implemented novel, cost-effective, high-performance cloud computing to reveal architectural features in unprecedented detail. We analyzed ADDomer interaction with components of the immune system and developed a promising first-in-kind ADDomer-based vaccine candidate to combat emerging Chikungunya infectious disease, exemplifying the potential of our approach.

Original language	English
Article number	eaaw2853
Number of pages	9
Journal	Science Advances
Volume	5
Issue number	9
DOIs	https://doi.org/10.1126/sciadv.aaw2853
Publication status	Published - 25 Sept 2019

Research Groups and Themes

Bristol BioDesign Institute
BrisSynBio

Keywords

SYNTHETIC BIOLOGY
minimal biology
Biodesign
Vaccine
advanced computing

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1126/sciadv.aaw2853

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Vragniau, C., Bufton, J. C., Garzoni, F., Stermann, E., Rabi, F., Terrat, C., Guidetti, M., Josserand, V., Williams, M., Woods, C. J., Viedma, G., Bates, P., Verrier, B., Chaperot, L., Schaffitzel, C., Berger, I., & Fender, P. (2019). Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display. Science Advances, 5(9), Article eaaw2853. https://doi.org/10.1126/sciadv.aaw2853

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title = "Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display",

abstract = "Self-assembling virus-like particles represent highly attractive tools for developing next-generation vaccines and protein therapeutics. We created ADDomer, an adenovirus-derived multimeric protein-based self-assembling nanoparticle scaffold engineered to facilitate plug-and-play display of multiple immunogenic epitopes from pathogens. We used cryo–electron microscopy at near-atomic resolution and implemented novel, cost-effective, high-performance cloud computing to reveal architectural features in unprecedented detail. We analyzed ADDomer interaction with components of the immune system and developed a promising first-in-kind ADDomer-based vaccine candidate to combat emerging Chikungunya infectious disease, exemplifying the potential of our approach.",

keywords = "SYNTHETIC BIOLOGY, minimal biology, Biodesign, Vaccine, advanced computing",

author = "Charles Vragniau and Bufton, {Joshua C.} and Fr{\'e}d{\'e}ric Garzoni and Emilie Stermann and Fruzsina Rabi and C{\'e}line Terrat and M{\'e}lanie Guidetti and V{\'e}ronique Josserand and Matt Williams and Woods, {Christopher J.} and Gerardo Viedma and Phil Bates and Bernard Verrier and Laurence Chaperot and Christiane Schaffitzel and Imre Berger and Pascal Fender",

year = "2019",

month = sep,

day = "25",

doi = "10.1126/sciadv.aaw2853",

language = "English",

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Vragniau, C, Bufton, JC, Garzoni, F, Stermann, E, Rabi, F, Terrat, C, Guidetti, M, Josserand, V, Williams, M, Woods, CJ, Viedma, G, Bates, P, Verrier, B, Chaperot, L, Schaffitzel, C , Berger, I & Fender, P 2019, 'Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display', Science Advances, vol. 5, no. 9, eaaw2853. https://doi.org/10.1126/sciadv.aaw2853

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T1 - Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display

AU - Vragniau, Charles

AU - Bufton, Joshua C.

AU - Garzoni, Frédéric

AU - Stermann, Emilie

AU - Rabi, Fruzsina

AU - Terrat, Céline

AU - Guidetti, Mélanie

AU - Josserand, Véronique

AU - Williams, Matt

AU - Woods, Christopher J.

AU - Viedma, Gerardo

AU - Bates, Phil

AU - Verrier, Bernard

AU - Chaperot, Laurence

AU - Schaffitzel, Christiane

AU - Berger, Imre

AU - Fender, Pascal

PY - 2019/9/25

Y1 - 2019/9/25

N2 - Self-assembling virus-like particles represent highly attractive tools for developing next-generation vaccines and protein therapeutics. We created ADDomer, an adenovirus-derived multimeric protein-based self-assembling nanoparticle scaffold engineered to facilitate plug-and-play display of multiple immunogenic epitopes from pathogens. We used cryo–electron microscopy at near-atomic resolution and implemented novel, cost-effective, high-performance cloud computing to reveal architectural features in unprecedented detail. We analyzed ADDomer interaction with components of the immune system and developed a promising first-in-kind ADDomer-based vaccine candidate to combat emerging Chikungunya infectious disease, exemplifying the potential of our approach.

AB - Self-assembling virus-like particles represent highly attractive tools for developing next-generation vaccines and protein therapeutics. We created ADDomer, an adenovirus-derived multimeric protein-based self-assembling nanoparticle scaffold engineered to facilitate plug-and-play display of multiple immunogenic epitopes from pathogens. We used cryo–electron microscopy at near-atomic resolution and implemented novel, cost-effective, high-performance cloud computing to reveal architectural features in unprecedented detail. We analyzed ADDomer interaction with components of the immune system and developed a promising first-in-kind ADDomer-based vaccine candidate to combat emerging Chikungunya infectious disease, exemplifying the potential of our approach.

KW - SYNTHETIC BIOLOGY

KW - minimal biology

KW - Biodesign

KW - Vaccine

KW - advanced computing

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VL - 5

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Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display

Abstract

Research Groups and Themes

Keywords

UN SDGs

Access to Document

Handle.net

Other files and links

Embargoed Document

Fingerprint

Membrane protein insertion and quality control by the bacterial holo-translocon and FtsH chaperone/protease complex

BrisSynBio: Bristol Centre for Synthetic Biology

Cite this

Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display

Abstract

Research Groups and Themes

Keywords

UN SDGs

Access to Document

Handle.net

Other files and links

Embargoed Document

Fingerprint

Projects

Membrane protein insertion and quality control by the bacterial holo-translocon and FtsH chaperone/protease complex

BrisSynBio: Bristol Centre for Synthetic Biology

Cite this